Addgene

We narrowed to 19,014 results for: tes

Showing: 4201 - 4220 of 19014 results

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LentiCRISPR v2 sgZER1-1

Plasmid

#191549

Purpose

Expression of spCas9 and sgRNA targetting ZER1

Depositor

Elizabeth White

Article

Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

Insert

spCas9 (ZER1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2 sgZER1-2

Plasmid

#191550

Purpose

Expression of spCas9 and sgRNA targetting ZER1

Depositor

Elizabeth White

Article

Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

Insert

spCas9 (ZER1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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SpAGS-dGoLoco1-GFP

Plasmid

#185577

Purpose

for overexpression

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

AGS-dGoLoco1

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Expression

Mutation

deletion of GoLoco motif #1 (aa473 DNFFEALSRFQSNR…

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2Aq(2nd)

Plasmid

#183089

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pUC-TalO8 (Blank-CoTrIP)

Plasmid

#178303

Purpose

Blank CoTrIP plasmid

Depositor

Brian Zid

Article

Chen et al Elife. 2022 Sep 15;11. pii: 76965. doi:

Type

Empty backbone

Use

Tags

NA

Expression

Yeast

Mutation

Promoter

NA

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NK88

Plasmid

#176612

Purpose

To manipulate ligand spacing.

Depositor

Meghan Morrissey

Article

Kern et al Elife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

DNA-CARy- aTM (aa291-404)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

n/a

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCup_771-1132_C

Plasmid

#146030

Purpose

Insect Expression of DmCup_771-1132

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_771-1132 (cup Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup_771-1132_C

Plasmid

#146034

Purpose

Insect Expression of DmCup_771-1132

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_771-1132 (cup Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup-Y342AL347A_C

Plasmid

#146035

Purpose

Insect Expression of DmCup-Y342AL347A

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup-Y342AL347A (cup Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSA293-CHEQ-seq

Plasmid

#174669

Purpose

Reporter plasmid for MPRA containing a SCP1 promoter, a chimeric intron and the Venus cDNA.

Depositor

Stein Aerts

Article

Mauduit et al Elife. 2021 Dec 7;10. pii: 71735. doi: 1

Type

Empty backbone

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

SCP1

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJH805_pJH_LldR_Pcon-sfGFP.0TAG

Plasmid

#163736

Purpose

Constitutive expression of green fluorescent protein encoding zero stop codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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U6-U57A

Plasmid

#169927

Purpose

WT U6 with a U57A mutation

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

U6 U57A

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-ORF68 (tagless)

Plasmid

#166025

Purpose

Expresses ORF68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-EnRRbpj

Plasmid

#160925

Purpose

dominant-negative construct to inhibit Notch signaling

Depositor

Mark Emerson

Article

Chen et al Sci Rep. 2021 Jul 15;11(1):14525. doi: 1

Insert

EnRRbpj

Use

Tags

Expression

Mutation

fused with an engrailed repressor

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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p8208 MSCV-Neo C-HA EMPTY

Plasmid

#163310

Purpose

Empty vector control

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLL3.7-Rictor-shRNA

Plasmid

#157930

Purpose

Knockdown of Rictor

Depositor

Baoji Xu

Article

Xu et al Nat Commun. 2019 Aug 9;10(1):3622. doi:

Insert

Rictor shRNA

Use

Lentiviral and RNAi

Tags

tdTomato

Expression

Mutation

Promoter

mouse U6

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-clbH-A1

Plasmid

#114149

Purpose

Expresses ClbH-A1

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbH-A1

Use

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Contains only the A1 domain of ClbH

Promoter

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pre-Ubc4 BrG pUC19

Plasmid

#111322

Purpose

for transcription to Ubc4 pre-mRNA

Depositor

Christine Guthrie

Article

Abelson et al Methods Enzymol. 2010;472:31-40. doi: 10

Insert

UBC4 (UBC4 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

intron has branch-site A to G mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 dATG1 (1188)

Plasmid

#66918

Purpose

bacterial expression of dATG1

Depositor

Michael Karin, Jun Hee Lee

Article

Kim et al Autophagy. 2013 Aug;9(8):1201-13. doi: 1

Insert

ATG1

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pAP757-1

Plasmid

#99493

Purpose

6XHis::TEV::3XFlag::linker::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

6XHis::TEV::3XFlag::linker::Myc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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