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Plasmid#180338Purposemammalian expression of human SEPT9_i1 S22A S23A fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v1 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT9_i1 S22A S23APromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1 R10A R15A-msfGFP
Plasmid#180336Purposemammalian expression of human SEPT9_i1 R10A R15A fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v1 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT9_i1 R10A R15APromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1 S12A S13A-msfGFP
Plasmid#180337Purposemammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v1 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT9_i1 S12A S13APromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
p-sfGFP-pA-FRT-Amp-FRT
Plasmid#176620Purposefor generating PCR templates with sfGFP and Amp for BAC recombinationDepositorInsertsuper folder green fluorescent protein with FRT-flanked ampicillin cassette
UseUnspecifiedTagsExpressionMutationH217R - please see dep. commentsPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xQUAS-sfGFP-MiniWhite
Plasmid#165922PurposeBlasticidin resistant 5xQUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable QUAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-DogTag Loop C
Plasmid#171781PurposeExpresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp173 and Gly174DepositorInsertsfGFP-DogTag Loop C
UseTagsHis6ExpressionBacterialMutationDogTag and linkers inserted between residues Asp1…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-SpyTag003 Loop B
Plasmid#171777PurposeExpresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp102 and Asp103DepositorInsertsfGFP-SpyTag003 Loop B
UseTagsHis6ExpressionBacterialMutationSpyTag003 and linkers inserted between residues A…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-SpyTag003 Loop C
Plasmid#171778PurposeExpresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp173 and Gly174DepositorInsertsfGFP-SpyTag003 Loop C
UseTagsHis6ExpressionBacterialMutationSpyTag003 and linkers inserted between residues A…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-12xLexAOp-sfGFP-MiniWhite
Plasmid#165916PurposeBlasticidin resistant 12xLexAOp sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable LexAOp Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-R-bpA-3xSV40pA-R-td-sfGFP
Plasmid#133579PurposeCAG tdGFP dre/rox reporter piggyBac transposon (bpA 3x SV40pA stop)DepositorInsertTandem dimer superfolder GFP
UseDre/roxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-R-bpA-2xSV40pA-R-td-sfGFP
Plasmid#133578PurposeCAG tdGFP dre/rox reporter piggyBac transposon (bpA 2x SV40pA stop)DepositorInsertTandem dimer superfolder GFP
UseDre/roxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-F-bpA-SV40pA-F-td-sfGFP
Plasmid#133571PurposeCAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 1x SV40pA stop)DepositorInsertTandem dimer superfolder GFP
UseFlp/frtTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW yosfGFP-T2A-mCherry
Plasmid#118448PurposeProduces equimolar expression of yeast codon-optimized sfGFP and mCherryDepositorInsertyosfGFP-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMC-J6-NbGP41-sfGFP-VP64-GB1-J9
Plasmid#202007PurposeMoClo level 0 NbGP41-sfGFP-VP64-GB1 coding sequence moduleDepositorInsertNbGP41-sfGFP-VP64-GB1
UseSynthetic Biology; Moclo cloning vectorTagsGB1 and sfGFPExpressionMutationPromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti sfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164478PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.DepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTagsmCherry and superfolder GFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
TfR-sfGFP-myc tag-SpyCatcher003
Plasmid#133451PurposeExpresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detectionDepositorInsertTfR-sfGFP-myc tag-SpyCatcher003
UseTagsGSSGS and myc tagExpressionMammalianMutationContains C20 and A23 mutations that improve plasm…PromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN-FKBP DD(L106P)-UbK0G75S-sfGFP
Plasmid#170157PurposeExpression for LIBRON constructsDepositorInsertFKBP DD (L106P)-UbK0G75S
UseRetroviralTagssfGFPExpressionMutationchange all lysin residues of ubiquitin to Arg, G7…PromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-tdMCP-msfGFP(V206K)-GBI
Plasmid#205163Purposeexpress tdMCP-msfGFP(V206K)-GBIDepositorInserttdMCP-msfGFP(V206K)-GBI
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SARS-CoV-2-S-RBD-sfGFP
Plasmid#141184PurposeMammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFPDepositorHas ServiceCloning Grade DNAInsertS surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
UseTagsSignal peptide from influenza HA and Superfolder …ExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mCherry-sfGFP-kanMX (pMaM17)
Plasmid#173452PurposeContains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP-ADH1ter
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only