Addgene

We narrowed to 2,326 results for: hells

Showing: 401 - 420 of 2326 results

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pXD71Cas10.4RT4-Pct5.1-crRNA(cgr2)-RT(Δcgr12)

Plasmid

#191650

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cgr2-targeting spacer) cgr1/cgr2-deleting repair template, used for cgr1/cgr2 deletion

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-NFIB-T2A-miRFP670

Plasmid

#187222

Purpose

Expresses human NFIB and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Nuclear Factor I B (NFIB Human)

Use

Tags

Inserted T2A-miRFP670 at 3' terminal of MCS.

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro

Plasmid

#198441

Purpose

Activity/inhibition assays, 19F-NMR

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A,

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-TVA-eGFP-N2cG

Plasmid

#175440

Purpose

Cre-on / Flp-off helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

Depositor

Gordon Fishell

Article

Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

Insert

TVA-P2A-eGFP-P2A-N2cG

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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gRNA_SOX2_HDR

Plasmid

#163752

Purpose

gRNA expression vector to target the SOX2 stop codon for HDR gene tagging in human cells.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

gRNA_SOX2 (SOX2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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hSyn-GW-dCas9-SET2-R195G-C201A-IRES2-mCherry

Plasmid

#202090

Purpose

Neuron-specific expression vector of catalytically-dead dCas9-Set2 containing R195G and C201A mutations

Depositor

Elizabeth Heller

Article

Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

Insert

dCas9-Set2(R195G, C201A)

Use

Tags

3x FLAG and mCherry

Expression

Mammalian

Mutation

R195G and C201A abolish catalytic activity

Promoter

human Synapsin

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23-Gja1prom

Plasmid

#188114

Purpose

Expresses luciferase under control of Gja1 promoter in transfected cells

Depositor

Jennifer Mitchell

Article

Shchuka et al PLoS One. 2023 Jan 3;18(1):e0271081. doi

Insert

Gja1 promoter (Gja1 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Gja1 endogenous promoter up to gene start codon

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W5F

Plasmid

#198443

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W5F

Promoter

T7 promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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5ZiPro

Plasmid

#198432

Purpose

Conformation sensitive site specific Cys-labeling

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

S84*C, R95*A, C80S, C143S, S160C, C178S,

Promoter

T7 promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W61*F

Plasmid

#198442

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

W61*F, R95*A,

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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eZiPro W5F

Plasmid

#198436

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

W5F

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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eZiPro W83F

Plasmid

#198439

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

W83F

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W89F

Plasmid

#198447

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W89F

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W83F

Plasmid

#198446

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W83F

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
eZiPro W69F

Plasmid

#198438

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

W69F

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
eZiPro W89F

Plasmid

#198440

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

W89F

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
gZiPro W69F

Plasmid

#198445

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W69F

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
eZiPro

Plasmid

#198434

Purpose

Activity/inhibition assays, 19F-NMR

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
eZiPro W61*F

Plasmid

#198435

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

W61*F

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
eZiPro W50F

Plasmid

#198437

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Tanja Schirmeister, Ute Hellmich

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

W50F

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge