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p663-UBC-miniTurbo-V5-CAMKK1_IDG-K
Plasmid#158169PurposeGateway destination clone of CAMKK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CAMKK1 (CAMKK1 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CDK7_IDG-K
Plasmid#158194PurposeGateway destination clone of CDK7 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CDK7 (CDK7 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CDK10_IDG-K
Plasmid#158171PurposeGateway destination clone of CDK10 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CDK10 (CDK10 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-2xV5-miniTurbo-NES-STOP_IDG-K
Plasmid#158161PurposeGateway destination clone of miniTurbo-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsert2xV5-miniTurbo-NES
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dzCas9-NG-Act3.0
Plasmid#158415PurposeCRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.DepositorInsertdzCas9-NG-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS18
Plasmid#158787PurposeRibosome-tagged GCaMP (soma localized) expression pan-neuronally C. elegansDepositorInsertPtag-168::GCaMP6m::rpl-1a
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO_FLAG.Ollas.VSVg_NGFR
Plasmid#158324PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsFLAG.Ollas.VSVgExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO_V5.AU1.VSVg_NGFR
Plasmid#158241PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsV5.AU1.VSVgExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-CTSB-blast
Plasmid#158464PurposeConstitutive lentiviral expression of human CTSB.DepositorInsertCTSB (CTSB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-CTSL
Plasmid#158444PurposeGateway Cloning compatible entry vector for the human CTSL gene.DepositorInsertCTSL (CTSL Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A_no_ccDB_mUty
Plasmid#158570PurposeV5-tagged sequence of mUty cloned into entry vector for recombinationDepositorInsertMus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mGold-Actin-C-18
Plasmid#158009PurposeVisualization of actin in mammalian cells using mGoldDepositorInsertActin (ACTB Human)
UseTagsmGoldExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pISH-Venus
Plasmid#15865DepositorInsertVenus
UseTagsExpressionMutationPromoterAvailable sinceMay 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαq*-BERKY1
Plasmid#158430PurposeBRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-GRK2(RH)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GFP-TAG
Plasmid#158131PurposeSingle fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)DepositorInsertsfGFP
UseTagsExpressionYeastMutationIntroduced a stop codon in place of the tyrosine …PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
ND4 left TALE–G1333 DddAtox C
Plasmid#158092Purposeexpresses the right-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertSOD2 MTS–3xHA–ND4 left TALE–G1333 DddAtox-C–1xUGI
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-EYFP-ccdB-G418
Plasmid#158550PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEYFPExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-DPP4
Plasmid#158439PurposeGateway Cloning compatible entry vector for the human DPP4 gene.DepositorInsertDPP4 (DPP4 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
MAC-SARS-CoV-2 NSP2
Plasmid#158353PurposeMAC-tagged gene expressionDepositorInsertSARS-CoV-2 NSP2 (ORF1ab )
UseTagsMAC-tagExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits
