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Showing: 1001 - 1050 of 1188 results

  1. pMTB-Multibow-hR

    Plasmid
    #60997
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear dTomato
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    dTomato
    Use
    Zebrafish
    Tags
    histone 2B tag
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    March 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. pMTB-Multibow-hG

    Plasmid
    #60994
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. . Encodes nuclear EGFP
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    EGFP
    Use
    Zebrafish
    Tags
    histone 2B tag
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Jan. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pMTB-Multibow-hC

    Plasmid
    #60993
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear cerulean
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    Cerulean
    Use
    Zebrafish
    Tags
    histone 2B tag
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pMTB-Multibow-hfR

    Plasmid
    #60998
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. . Encodes nuclear mKate2.
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    mKate2
    Use
    Zebrafish
    Tags
    histone 2B tag
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Jan. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pMTB-Multibow-hO

    Plasmid
    #60996
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear mKO
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    mKO
    Use
    Zebrafish
    Tags
    histone 2B tag
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Jan. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pCL1177

    Plasmid
    #217961
    Purpose
    Dual cistronic expression vector for the RAPID-release approach. H3.2-mCherry is released from mitochondria to the nucleus upon addition of rapamycin.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H3 (HIST2H3PS2 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217961-plasmid-map-sequence-id-430215

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  13. pCS2+ H2B-mCherry

    Plasmid
    #99265
    Purpose
    Contains a fusion of human histone H2B to fluorescent protein mCherry in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.
    Depositor
    Philipp Keller
    Article
    Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi
    Insert
    mCherry
    Use
    Tags
    H2B
    Expression
    Mammalian
    Mutation
    Promoter
    SP6
    Available since
    Sept. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    99265-plasmid-map-sequence-id-192948

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  19. pMH-macroHC

    Plasmid
    #82337
    Purpose
    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli
    Depositor
    Michael Hothorn
    Article
    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p
    Insert
    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    Use
    Tags
    6xHis non-cleavable and macro domain of human his…
    Expression
    Bacterial
    Mutation
    Truncated protein, amino acids 1-178
    Promoter
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    82337-plasmid-map-sequence-id-155830

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  21. pAGH51

    Plasmid
    #107255
    Purpose
    HU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.
    Depositor
    Erin O'Shea
    Article
    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111
    Insert
    HU-FRB
    Use
    Synthetic Biology
    Tags
    FRB
    Expression
    Mutation
    Promoter
    native HU promoter
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107255-plasmid-map-sequence-id-207562

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  22. pCAG-HDAC4-IGd2

    Plasmid
    #22536
    Purpose
    To be used for the RNAi screening purposes (not for HDAC4 expression); see paper
    Depositor
    Connie Cepko
    Article
    Chen et al Science. 2009 Jan 9. 323(5911):256-9.
    Insert
    Histone Deacetylase 4 (HDAC4 Human)
    Use
    Tags
    IGd2 = Ires-destabilized GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 9, 2009
    Availability
    Academic Institutions and Nonprofits only

  33. pCAG-nucGFP11-3xControlgRNA

    Plasmid
    #224568
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    GFP11-H2B
    Use
    CRISPR
    Tags
    Histone H2B (nuclear localization)
    Expression
    Mutation
    Codon optimized
    Promoter
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224568-plasmid-map-sequence-id-451496

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  38. pJK2020

    Plasmid
    #171932
    Purpose
    express MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operon
    Depositor
    Judith Kimble
    Article
    Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.
    Insert
    Pmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR
    Use
    Tags
    superfolder GFP, mCherry
    Expression
    Worm
    Mutation
    Promoter
    -
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    171932-plasmid-map-sequence-id-337179

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  39. pH2B-SCFP1-2A-mTq2-NES

    Plasmid
    #60491
    Purpose
    Co-expresses two cyan fluorescent protein variants, a short fluorescence lifetime CFP variant in the nucleus and a CFP variant with a long lifetime (mTurquoise2) in the cytoplasm
    Depositor
    Dorus Gadella
    Article
    Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.
    Inserts
    H2B-SCFP1
    mTurquoise2-NES
    Use
    Tags
    SCFP1 and mTurquoise2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60491-plasmid-map-sequence-id-96973

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  50. pHis-parallel1-NLSH2BCas9

    Plasmid
    #112065
    Purpose
    Expression and purification of NLSH2BCas9 for fungal gene editing
    Depositor
    Jeff Coleman
    Article
    Wang et al Fungal Genet Biol. 2018 May 12. pii: S10
    Insert
    NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
    Use
    CRISPR
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Cas9 was codon optimized for Aspergillus niger
    Promoter
    promoter in vector
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    112065-plasmid-map-sequence-id-217190

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Showing: 1001 - 1050 of 1188 results