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Plasmid#192681PurposeLentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcriptionDepositorInsertMouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gαi*-BERKY1
Plasmid#158090PurposeBRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-YFP-KB1753-myc
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gαi*-BERKY2
Plasmid#158197PurposeBRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-2xYFP-KB1753-myc
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 AT1R DRY-AAY
Plasmid#45759DepositorInsertAT1R DRY-AAY (Agtr1a Rat)
UseTagsHAExpressionMammalianMutationchanged amino acids 125-126 from DR to AAPromoterCMVAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJCC_058 FnCas12a D917A
Plasmid#171667PurposeFor bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tagDepositorInsertFnCas12a D917A
UseTags6X His and sortase A sorting motifExpressionBacterialMutationchanged aspartate 917 to alaninePromoterAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pITGFP89
Plasmid#83560PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.DepositorInsertTEF1 promoter -yEGFP-CLN2PEST
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRE147
Plasmid#118499Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmClover3
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-NLS-dMisCas13b-3xGFP-NLS
Plasmid#191367PurposeOverexpression of NLS-NLS-dMisCas13b-3xGFP-NLS in human cellsDepositorInsertNLS-NLS-dMisCas13b-3xGFP-NLS
UseTags3xGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P01
Plasmid#120012PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFPDepositorInsertmoxBFP
UsePcr templateTagsmoxBFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P27
Plasmid#120038PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 1xHADepositorInsert1xHA
UsePcr templateTags1xHAExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDisplay FLIPE-1m
Plasmid#13548DepositorInsertFLIP-E A207W
UseTagsECFP and VenusExpressionMammalianMutationA207W in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGL3_4xUAS_CP-candidate_luc+
Plasmid#125154PurposeSTAP-seq luciferase validation vector, which harbors an 4x UAS array upstream of the CP position (candidates are inserted at CP position) for the recruitement of GAL4-DBD tagged proteinsDepositorInsert4 x upstream activating sequence (UAS)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLIPE-10u
Plasmid#13542DepositorInsertFLIP-E A207R
UseTagsECFP and VenusExpressionMammalianMutationA207R in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pM116: CasRx VEGFA presgRNA
Plasmid#166868PurposeU6-driven expression of human VEGFA targeting presgRNA compatible with CasRx.DepositorInsertHuman VEGFA targeting presgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-mMcl-1
Plasmid#32980DepositorInsertMcl-1 (Mcl1 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEE495
Plasmid#149291PurposeT-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2DepositorInsertp35S:TRV2_Direct_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Septin 11 isoform IV
Plasmid#120320PurposeSeptin 11 isoform IV mammalian expressionDepositorInsertSEPT11 (Sept11 Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Septin 11 isoform I
Plasmid#120314PurposeSeptin 11 isoform I mammalian expressionDepositorInsertSEPT11 (Sept11 Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRE157
Plasmid#118507Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmClover3
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Myc-SH3BP4
Plasmid#46890Purposeexpresses Myc tagged SH3BP4 in mammalian cellsDepositorInsertSH3BP4 (SH3BP4 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only