Addgene

We narrowed to 15,233 results for: LEA

Showing: 4041 - 4060 of 15233 results

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p2T-CMV-eA3Amax-T31A-NG-BlastR

Plasmid

#153000

Purpose

C-to-T/G/A base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-BE4 T31A-nSpCas9-UGI-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pMJ823

Plasmid

#39313

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9 (lin2744 Listeria innocua)

Use

CRISPR

Tags

MBP and thioredoxin-His6-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-mPCSK7

Plasmid

#122676

Purpose

Expresses mouse PCSK7 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus proprotein convertase subtilisin/kexin type 7 (Pcsk7), transcript variant 2 (Pcsk7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-ERK2 T183AY185F

Plasmid

#118299

Purpose

Expression of Rat ERK2

Depositor

Philip Stork

Article

Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

Insert

ERK2 T183AY185F (Mapk1 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

T183AY185F

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-eA3AmaxNG-BlastR

Plasmid

#152999

Purpose

C-to-T base editor, NG PAM

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-nSpCas9 NG-UGI-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-GRAB_g5-HT3.0mut

Plasmid

#208724

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al bioRxiv 2023.05.27.542566

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPE-1m

Plasmid

#13544

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207W in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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HS-PCNA-GFP

Plasmid

#105942

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

PCNA (PCNA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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YTIP-GFP

Plasmid

#42238

Depositor

William Pu

Article

von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

Insert

YTIP-GFP

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-36α

Plasmid

#214343

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL36A (IL36A Human)

Use

Tags

His-TEV and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pOCC151

Plasmid

#118899

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal GST, cleavable with 3C

Depositor

PEPC MPI CBG, Marc Bickle

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-GST-3C-NotI-ccdB-AscI-stop-HindIII cassette

Use

Tags

GST tag, cleavable with 3C protease

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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3xEGFP-mMeikin

Plasmid

#174708

Purpose

Mammalian expression of mouse Meikin

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin (Meikin Mouse)

Use

Tags

3xEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pNW3

Plasmid

#53372

Purpose

Csy4 and Cas9 D10A nickase expression plasmid

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

Csy4-T2A-Cas9n (D10A)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

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pTP443

Plasmid

#216361

Purpose

xNup98-Nb1t (+1Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup98-Nb1t (+1Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSens-4OmeNB

Plasmid

#216231

Purpose

Expresses GFP in response to 4'-O-Methylnorbelladine

Depositor

Andrew Ellington, Simon d'Oelsnitz

Article

d'Oelsnitz et al Nat Commun. 2024 Mar 7;15(1):2084. doi:

Inserts

RamR-4NB2
sfGFP

Use

Synthetic Biology

Tags

Expression

Mutation

K64T, L67M, C135D, S138G

Promoter

J23103 and Pramr

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pNb4OMT-A53M

Plasmid

#216232

Purpose

Expresses a mutant Norbelladine 4'-O-Methyltransferase

Depositor

Andrew Ellington, Simon d'Oelsnitz

Article

d'Oelsnitz et al Nat Commun. 2024 Mar 7;15(1):2084. doi:

Insert

Nb4OMT-A53M

Use

Synthetic Biology

Tags

Expression

Mutation

A53M

Promoter

P150

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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Pet15b-Internalization Control

Plasmid

#186553

Purpose

Negative Control mediating endosomal localization

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

10R-8His-EGFP

Use

Tags

8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pRG710_lexO-HO_LexA-TF_HIS3MX

Plasmid

#154819

Purpose

Inducible expression of HO endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

HO endonuclease (HO Budding Yeast)
LexA-ER-B112

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

ACT1 and lexO_4-CYC1_core

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-GRAB_r5-HT1.0

Plasmid

#208725

Purpose

Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al bioRxiv 2023.05.27.542566

Insert

Red fluorescent 5-HT sensor GRAB_r5-HT1.0

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA

Plasmid

#178868

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVcs-mTagBFP-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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