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Addgene

GFP-ERK2 T183AY185F
(Plasmid #118299)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118299 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    peGFP-C1
  • Backbone manufacturer
    clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ERK2 T183AY185F
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1077
  • Mutation
    T183AY185F
  • Entrez Gene
    Mapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ERK2 constructs were subcloned into pEGFP-C1 with XhoI and BamHI (GFP-XhoI-ERK-BamHI)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-ERK2 T183AY185F was a gift from Philip Stork (Addgene plasmid # 118299 ; http://n2t.net/addgene:118299 ; RRID:Addgene_118299)
  • For your References section:

    Examining the mechanism of Erk nuclear translocation using green fluorescent protein. Horgan AM, Stork PJ. Exp Cell Res. 2003 May 1;285(2):208-20. S0014482703000375 [pii] PubMed 12706116