Addgene

We narrowed to 11,160 results for: 110

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pcDNA3-RIPK3[S199A][S227A]-FLAG

Plasmid

#199323

Purpose

double mutated phosphorylation site (kinase acitivity and MLKL interaction inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 and 227 to Alanine

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA_DiCre_60.F2

Plasmid

#60208

Purpose

Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343)

Depositor

Jean-Paul Herman

Article

Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

Insert

Cre recombinase (CD207 Synthetic, Bacteriophage P1, Human)

Use

Tags

FRAP and Nuclear localisation signal

Expression

Mammalian

Mutation

amino acids 2021-2112 of FRAP with a T2098L mutat…

Promoter

CMV

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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MBC1-PuroIII

Plasmid

#153419

Purpose

MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genes

Depositor

Maki Nakayama

Article

Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Clim-DC2-NMM-tdtomato

Plasmid

#129557

Purpose

pClimDC-NMM:tdT cell membrane marker

Depositor

Inaki Ruiz-Trillo

Article

Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

Insert

N-Myristoylation motif (NMM) fused to tdtomato

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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GST-GAR

Plasmid

#34697

Depositor

Steven Clarke

Article

Frankel et al J Biol Chem. 2000 Oct 20;275(42):32974-8

Insert

Fibrillarin (FBL Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Residues 1-145 of human fibrillarin with K2E and …

Promoter

tac

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only
aav-CAG-FLEX-tdNfsB(F124W)-mCherry (eNTR)

Plasmid

#113762

Purpose

AAV-mediated conditional expression of bacterial tdNfsB-mCherry (eNTR) under the CAG promoter

Depositor

Luke Lavis

Article

Gruber et al ACS Chem Biol. 2018 Aug 29. doi: 10.1021

Insert

tdNfsB(F124W)-mCherry

Use

AAV and Cre/Lox

Tags

mCherry

Expression

Mammalian

Mutation

F124W

Promoter

CAG

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCIG-Flag-Sox9-IRES-nls-GFP

Plasmid

#44281

Depositor

Martin Cheung

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

Insert

Flag-Sox9 (SOX9 Chicken)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV-IE enhancer and Chicken beta-actin promoter

Available since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only
pMALp2x-SCO5461(43-204)

Plasmid

#42509

Depositor

Koji Okamoto

Article

Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

Insert

guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))

Use

Tags

maltose-binding protein

Expression

Bacterial

Mutation

deleted amino acids 1-42 (nucleotide 1-126)

Promoter

tac

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
Bin1 N-BAR domain

Plasmid

#168280

Purpose

Express N-BAR domain of Bin1 (aa 1-267) in pEGFP-N1 vector

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

Bin1 (Bin1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-267

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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BtGRK2-sYFP2

Plasmid

#137778

Purpose

Visualization of GRK2

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Bovine GRK2 (GRK2 Bovine)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pet23a_RBPMS_FL

Plasmid

#59392

Purpose

Plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP

Plasmid

#103815

Purpose

Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN (CIB1 Mustard Weed)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-SID4X-pU6-sgRNA

Plasmid

#158989

Purpose

Vector F encodes pAAV-pMecp2-dSaCas9-SID4X-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-SID4X

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pMecp2

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-DmrC

Plasmid

#61673

Purpose

Non-fluorescent, truncated kinesin-1 fused to DmrC linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-DmrC (Kif5c Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCfB2903(XI-2 MarkerFree)

Plasmid

#73275

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pSR357.9

Plasmid

#125105

Purpose

Expression of narL(1-129aa)-ydfI(124-213aa) chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-ydfI(DBD)129
sfgfp
tetR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Dynamin I-K44A-non-tagged

Plasmid

#32695

Depositor

Robert Lefkowitz

Article

Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

Insert

Dynamin I (Dnm1 Rat)

Use

Tags

Expression

Mammalian

Mutation

K44A

Promoter

CMV

Available since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pDONR221 DPP9S

Plasmid

#166789

Purpose

pDONR gateway shuttling vector with DPP9S (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

Promoter

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFPm-DAD M1041A

Plasmid

#25411

Depositor

Arthur Alberts

Article

Alberts et al J Biol Chem. 2001 Jan 26. 276(4):2824-30

Insert

Diap3 (Diaph3 Mouse)

Use

Tags

EGFP, His, and Myc

Expression

Mammalian

Mutation

Alanine substitution at critical residue in DAD t…

Promoter

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only
CFP-OSM

Plasmid

#12190

Depositor

Gary Johnson

Article

Uhlik et al Nat Cell Biol. 2003 Dec . 5(12):1104-10.

Insert

OSM (Ccm2 Mouse)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only