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Plasmid#66562PurposeEncodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.DepositorInsertCelTag
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sender plasmid
Plasmid#127677PurposeEncodes the synthase LuxI for the quorum-sensing molecule C6-HSL.DepositorInsertLuxI
UseSynthetic BiologyTagsLVAExpressionBacterialMutationPromoterpT7Available sinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV plasmid
Plasmid#169739PurposeUnregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Noodles Plasmid
Plasmid#212613PurposeAn all-in-one system for on-target efficiency analysis of CRISPR guide RNAsDepositorTypeEmpty backboneUseCRISPR and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Accessory plasmid 1
Plasmid#128569Purposefor mixing with SPECS library to produce lentivirusDepositorInsertECFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
Accessory plasmid 2
Plasmid#128571Purposefor cloning identified individual SPECSDepositorTypeEmpty backboneUseLentiviralTagsTagRFPExpressionMutationPromoterAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB4k5_RNAnano (r-plasmid)
Plasmid#127634PurposeEncodes an RNA nanostructure with MG, PP7, TAR and S1m RNA aptamers. Transcription via T7 RNAP.DepositorInsertRNA nanostrcutre
UseSynthetic BiologyTagsExpressionMutationPromoterT7 RNAPAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
BASU RaPID plasmid
Plasmid#107250PurposeExpresses the BASU RaPID labeling proteinDepositorInsertBASU
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tn7 helper plasmid
Plasmid#141161PurposeTemperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoterDepositorInsertTn7 transposon machinery
UseTagsExpressionBacterialMutationPromoterArabinose-inducible pBADAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
PAX6 donor plasmid
Plasmid#105239PurposeKnock in H2B-GFP into the human PAX6 locusDepositorInsertPAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette
UseTALENTagsExpressionMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
E.coli RaPID plasmid
Plasmid#107251PurposeExpresses the E.coli RaPID labeling proteinDepositorInsertE.coli BirA*
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
"AND-gate" receiver plasmid
Plasmid#127676PurposeEncodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.DepositorInsertLuxR, GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLlacO-1 for LuxR, pLux for GFPAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH29_32 series plasmid
Plasmid#120901PurposeAlso known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated deviceDepositorInsertsEc-TTL-P109
sRNA-A targeting sequence
RBS TIR=974 of GFPop1 gene
GFPop1
RBS TIR=6474 of ECF32mut gene
ECF32mut
T7 terminator
resource competitor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
BGGLO QPCR PLASMID
Plasmid#79905PurposeEncodes partial human gamma- and beta-globing cDNA for use as QPCR standard as published in PMID: 14592835..DepositorInsertHuman partial HBG and HBB gene
UseTagsExpressionMutationPromoterNo promoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3_YPet-PCP (y-plasmid)
Plasmid#127637PurposeEncodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.DepositorInsertYPet
UseSynthetic BiologyTagsRNA binding peptide: PP7 coat protein (PCP)ExpressionBacterialMutationPromoterpJ21306Available sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3_mTq-Tat (c-plasmid)
Plasmid#127635PurposeEncodes a fluorecent protein with an RNA binding peptide: mTurquoise2-tat.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme PlmJ and RBS BBa_B0034.DepositorInsertmTurquoise2
UseSynthetic BiologyTagsRNA binding peptide: PCP and RNA binding peptide:…ExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_plasmid_donor_RD
Plasmid#86551PurposeHomology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabainDepositorInsertATP1A1 (ATP1A1 Human)
UseCRISPR and TALEN; Co-selection via hdr using ouab…TagsExpressionMammalianMutationQ118R, N129DPromoterAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
ptetmCherry_prey_plasmid-2
Plasmid#34617DepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterptetAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
Reciever_plasmid2
Plasmid#140695PurposeEncodes quorum sensing (C4-HSL) inducible cyan (sfCFP) expressionDepositorInsertsfCFP
UseTagsExpressionBacterialMutationPromoterengineered rhl promoter (responds to C4-HSL)Available sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Receiver_plasmid1
Plasmid#140694PurposeEncodes constitutive red (mCherry) fluorescence for identificationDepositorInsertmCherry
UseTagsssrA degradation tag (ASV)ExpressionBacterialMutationPromoterpIq constitutive promoterAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only