1. lenti dCAS-VP64_Blast

   Plasmid

   #61425

   Purpose

   3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Lentiviral Prep

   Insert

   dCAS9(D10A, N863A)-VP64_2A_Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1A

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pTXBI-mClover-YTH domain

   Plasmid

   #177123

   Purpose

   Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (YTH domain) (YTHDC1 Human)
   

   Use

   Tags

   Mxe GyrA intein-Chitin binding domain for protein…

   Expression

   Bacterial

   Mutation

   YTH domain only (IDR1 and IDR2 deletion)

   Promoter

   T7

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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