These results may also be relevant.
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pGFP-mHP1beta
Plasmid#181901PurposeFluorescently tagged HP1betaDepositorInsertmHP1beta (Cbx1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-mHP1alpha
Plasmid#181900PurposeFluorescently tagged HP1alphaDepositorInsertmHP1alpha (Cbx5 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKB083
Plasmid#181952PurposeIPTG-inducible expression of PhoQDepositorInsertPhoQ; lacI (lacI PhoQ - Salmonella enterica subsp. enterica serovar Typhimurium; lacI - E. coli)
UseTagsExpressionBacterialMutationPromoterPhoQ-Ptac; lacI-PlacIqAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOT_1 - lenti-EFS-LTBR-2A-puro
Plasmid#181970PurposeExpresses human LTBR linked to puromycin resistance via P2A for lentiviral delivery.DepositorInsertLTBR (LTBR Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-NGLY1-Puro
Plasmid#181916PurposeLentiviral plasmid that directs the expression of NGLY1 in mammalian cells.DepositorInsertN-glycanase 1 (NGLY1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-GFP11-Actin
Plasmid#181966PurposeHuman beta actin tagged with GFP11.DepositorInsertGFP11-Actin (ACTB Human)
UseTagsGFP11ExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-CoilR-Cys
Plasmid#181989PurposeUsed to label proteins with the CoilE genetically encoded tagDepositorInsertCoilR-Cys
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_SPTLC1_C133W
Plasmid#181921Purpose3rd generation, Inducible lentiviral plasmid for expression of human mutant SPTLC1 C133WDepositorInsertHuman serine palmitoyltransferase, long chain base subunit 1 (SPTLC1) mutant C133W (SPTLC1 Human)
UseLentiviral; Mammalian expression, , doxycycline i…TagsExpressionMammalianMutationcysteine at position 133 of the coding region of …Promotertight TRE promoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC0091
Plasmid#181955PurposeBeta catenin reporter control M51 Super 8x (mutated TCF/LEF binding sites) FOPFlash with Gluc/ClucDepositorInsert7xFOP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-mHP1gamma
Plasmid#181902PurposeFluorescently tagged HP1gammaDepositorInsertmHP1gamma (Cbx3 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-mCherry-p115
Plasmid#181996PurposeMammalian expression vector encoding p115 RhoGEF tagged with mCherry.DepositorInsertmCherry-p115 (ARHGEF1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEm-CoilE-Actin
Plasmid#181993PurposeCoilE genetically encoded tag on mEmerald-Actin, to be used with CoilR labeled with a reporterDepositorInsertmEm-CoilE-Actin
UseTagsmEmeraldExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-GFP-mHP1alpha
Plasmid#181912PurposeBacterial protein expression vector for GFP-tagged HP1alphaDepositorInsertmHP1alpha (Cbx5 Mouse)
UseTagsGFPExpressionBacterialMutationPromoterT7Available sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOT_4 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-LTBR
Plasmid#181973PurposeExpresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.DepositorInsertFMC6.3-BBz CAR; LTBR (LTBR Human, Synthetic)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA TfR1-miniR
Plasmid#181986PurposeMiniR genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with MiniE labeled with a reporterDepositorInsertTfR1-MiniR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOT_3 - lenti-EFS-FMC6.3-28z-2A-puro-2A-LTBR
Plasmid#181972PurposeExpresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.DepositorInsertFMC6.3-28z CAR; LTBR (LTBR Human, Synthetic)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps
Plasmid#181956PurposeGenetically encoded FRET-based cAMP indicator fused to AKAP79.DepositorInsertAKAP79-(Ci/Ce)-Epac2-camps (AKAP5 Human)
UseTagsAKAP79, Cerulean (CFP), Citrine (YFP), and HIS ta…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMECP2-GFP
Plasmid#181904PurposeFluorescently tagged MECP2, GFPDepositorInsertMECP2 (Mecp2 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.TRE.HA.RUNX1A.IRES.dTomato.PGK.sfGFP.P2A.Tet3G
Plasmid#181977PurposeHuman RUNX1A gene overexpressionDepositorInsertRUNX1A (human) (RUNX1 Human)
UseLentiviralTagsHA (Human influenza hemagglutinin)ExpressionMutationPromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRB117.2
Plasmid#181949PurposeaTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuSDepositorInsertUseTagsmNeonGreenExpressionBacterialMutationSee Depositor Comments BelowPromotermNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only
