168,290 results

1. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV9)

   Viral Prep

   #44361-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Article

   Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

   Promoter

   Syn

   Tags

   mCherry (Cre-dependent)

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pAAV.TBG.PI.Null.bGH (AAV8)

   Viral Prep

   #105536-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV.TBG.PI.Null.bGH (#105536). In addition to the viral particles, you will also receive purified pAAV.TBG.PI.Null.bGH plasmid DNA. AAV vector with TBG promoter. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   TBG

   Available Since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV Retrograde)

   Viral Prep

   #44362-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Article

   Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

   Promoter

   Syn

   Tags

   mCherry (Cre-dependent)

   Available Since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

4. 3xHA-TurboID-NLS_pCDNA3

   Plasmid

   #107171

   Purpose

   expresses 3xHA-tagged TurboID in the mammalian nucleus

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV Retrograde)

   Viral Prep

   #44361-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Article

   Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

   Promoter

   Syn

   Tags

   mCherry (Cre-dependent)

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-CaMKIIa-hChR2(H134R)-EYFP (AAV9)

   Viral Prep

   #26969-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Lee et al Nature. 2010 Jun 10. 465(7299):788-92.

   Promoter

   CaMKII

   Tags

   EYFP

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-S5E2-dTom-nlsdTom (AAV1)

   Viral Prep

   #135630-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-S5E2-dTom-nlsdTom (#135630). In addition to the viral particles, you will also receive purified pAAV-S5E2-dTom-nlsdTom plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Tags

   dTomato

   Available Since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

8. ppyCAG_RNaseH1_WT

   Plasmid

   #111906

   Purpose

   Express RNASEH1 in mammalian cell.

   Depositor

   Xiang-Dong Fu

   Article

   Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

   Insert

   RNASEH1 (RNASEH1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   July 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-flex-taCasp3-TEVp (AAV5)

   Viral Prep

   #45580-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-flex-taCasp3-TEVp (#45580). In addition to the viral particles, you will also receive purified pAAV-flex-taCasp3-TEVp plasmid DNA. EF1a-driven, Cre-dependent, bicicstronic expression of designer pro-taCasp3 and TEVp. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Nirao Shah, Jim Wells

   Article

   Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

   Promoter

   EF1a

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

10. pLVX-Tet3G blasticidin

    Plasmid

    #128061

    Purpose

    Lentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV pCAG-FLEX-EGFP-WPRE (AAV5)

    Viral Prep

    #51502-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Promoter

    CAG

    Tags

    EGFP (Cre-dependent)

    Available Since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

13. pLV-mCherry

    Plasmid

    #36084

    Purpose

    3rd generation lentiviral plasmid expressing mCherry

    Depositor

    Pantelis Tsoulfas

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO-GAlpha12-RLuc8

    Plasmid

    #140985

    Purpose

    Encodes a G alpha subunit (GNA12) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha12-RLuc8 (GNA12 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKrox24(MapErk)DsRed

    Plasmid

    #200114

    Purpose

    Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    MapErk sequence in minimal promoter
    

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    5 copies of designed MapErk sequence, no other pr…

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENN.AAV.CMVs.Pl.Cre.rBG (AAV9)

    Viral Prep

    #105537-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    CMV

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV1)

    Viral Prep

    #104492-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Promoter

    Syn

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pLIX_403

    Plasmid

    #41395

    Purpose

    Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    V5

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT/TO-GAlpha11-RLuc8

    Plasmid

    #140983

    Purpose

    Encodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha11-RLuc8 (GNA11 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mOrange2-C1

    Plasmid

    #54650

    Purpose

    Localization: C1 Cloning Vector, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson, Roger Tsien

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Tags

    mOrange2

    Expression

    Mammalian

    Available Since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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