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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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175,948 results

Showing: 21 - 40 of 175948 results

  1. L4440

    Plasmid
    #1654
    Depositor
    Andrew Fire
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    RNAi
    Tags
    T7p, T7p, lacZN, OriF1>>, OriF1<<
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1654-plasmid-map-sequence-id-422970

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  2. Flag-TurboID

    Plasmid
    #124646
    Purpose
    Expresses Flag-TurboID fusion for proximity labeling
    Depositor
    FENG-QIAN Li, Ken-Ichi Takemaru
    Type
    Empty backbone
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    124646-plasmid-map-sequence-id-435118

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  5. pAAV-hSyn-hM3D(Gq)-mCherry

    Plasmid
    #50474
    Purpose
    Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM3D(Gq)-mCherry (CHRM3 Human)
    Use
    AAV
    Tags
    mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50474-plasmid-map-sequence-id-471606

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  7. lentiCRISPR v2-Blast

    Plasmid
    #83480
    Purpose
    Mammalian expression of Cas9 and sgRNA scaffold
    Depositor
    Mohan Babu
    Insert
    Blasticidin S deaminase
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    EF-1α
    Available Since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83480-plasmid-map-sequence-id-423158

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  9. Human sgRNA library Brunello in lentiCRISPRv2 (Lentiviral Prep)

    Viral Prep
    #73179-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiCRISPRv2 (#73179). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2 plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. This backbone contains SpCas9 and unique gRNAs, and can be used to make edits across 19,114 genes in the human genome. In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2.
    Depositor
    John Doench, David Root
    Article
    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103
    Available Since
    June 14, 2017
    Availability
    Academic Institutions and Nonprofits only

  10. myc-BioID2-MCS

    Plasmid
    #74223
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    Myc and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    74223-plasmid-map-sequence-id-334595

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  19. Flag-miniTurbo

    Plasmid
    #124647
    Purpose
    Expresses Flag-miniTurbo fusion for proximity labeling
    Depositor
    FENG-QIAN Li, Ken-Ichi Takemaru
    Type
    Empty backbone
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    124647-plasmid-map-sequence-id-242444

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Showing: 21 - 40 of 175948 results