171,388 results
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Plasmid#174820PurposeMammalian expression of SpCas9 PEmax prime editorDepositorInsertPEmax
TagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSV-Rev
Plasmid#12253Purpose3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)DepositorAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/8
Plasmid#112864PurposeAAV packaging plasmid expressing Rep/Cap genesDepositorInsertRep2/Cap8
UseAAVPromoterTruncated P5Available SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-tevopreq1-GG-acceptor
Plasmid#174038PurposePrime editing in mammalian cellsDepositorTypeEmpty backboneExpressionMammalianPromoterhU6Available SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6b
Plasmid#207852PurposeMammalian expression of PE6b prime editorDepositorInsertPE6b
TagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationTf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…Available SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
scramble shRNA
Plasmid#1864Purpose3rd gen lentiviral negative control vector containing scrambled shRNADepositorInsertscramble
UseLentiviral and RNAiExpressionMammalianAvailable SinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1
Plasmid#20061PurposeBacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formationDepositorInsertMSP1D1
Tags7-His tagExpressionBacterialMutationdeletion mutant (1-11) of MSP1; His-tag on N-term…Available SinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_WT
Plasmid#83482PurposeBacterial expression of WT L. buccalis C2c2 CRISPR effector.DepositorInsertLbu_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialPromoterT7Available SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a-BirA
Plasmid#20857DepositorInsertBirA
Tags6xHisExpressionBacterialAvailable SinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E3D1
Plasmid#20066PurposeBacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formationDepositorInsertMSP1E3D1
Tags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeats of …Available SinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMDLg/pRRE
Plasmid#12251Purpose3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)DepositorInsertHIV-1 GAG/POL
UseLentiviral; PackagingExpressionMammalianAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDY1330 STITCHR pCMV-nCas9-XTEN-v170_R2Tocc
Plasmid#234826PurposeSTITCHR R2Tocc editorDepositorInsertnCas9h840a-xten-R2Tocc
UseCRISPRAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FlpO
Plasmid#89574PurposeExpresses FlpO under pCAGDepositorInsertFlpO
UseCre/Lox and Synthetic BiologyTagsNLSExpressionMammalianAvailable SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO mTRPM7
Plasmid#45482DepositorInsertTRPM7 (Trpm7 Mouse)
UseTetracycline inducibleTagsFlagExpressionMammalianPromoterTetracycline-controlled CMVAvailable SinceMay 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)
Plasmid#140003PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHN781
Plasmid#178828PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
TagsHis8, TEVExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available SinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
NW50
Plasmid#133445PurposeCovalently circularized nanodisc NW50 for studying membrane proteins and viral entry.DepositorInsertNW50
ExpressionBacterialAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1delltaH5
Plasmid#71714PurposeMembrane scaffold protein (MSP), helix 5 deletionDepositorAvailable SinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-LaminB1-10
Plasmid#55069PurposeLocalization: Nuclear Envelope, Excitation: 587, Emission: 610DepositorAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOS008: GCaMP6F calcium sensor (cytosolic)
Plasmid#163045PurposeGCaMP6F calcium sensor (cytosolic)DepositorInsertGCaMP6F
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only