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179,538 results

Showing: 21 - 40 of 179538 results

  1. pSpCas9n(BB)-2A-GFP (PX461)

    Plasmid
    #48140
    Purpose
    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA
    Depositor
    Feng Zhang
    Publication
    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9n
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Mutation
    Cas9 D10A nickase mutant
    Promoter
    Cbh
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    170 Articles
    48140-plasmid-map-sequence-id-334358

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  2. pCMV-dR8.2 dvpr

    Plasmid
    #8455
    Purpose
    2nd* generation lentiviral packaging plasmid. (*See comments section.) Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.
    Depositor
    Bob Weinberg
    Publication
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    493 Articles
    8455-plasmid-map-sequence-id-370240

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  3. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

    Plasmid
    #42335
    Purpose
    A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.
    Depositor
    Feng Zhang
    Publication
    Cong et al Science. 2013 Jan 3.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Available since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    227 Articles
    42335-plasmid-map-sequence-id-275689

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  7. M51 Super 8x FOPFlash (TOPFlash mutant)

    Plasmid
    #12457
    Purpose
    Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.
    Depositor
    Randall Moon
    Publication
    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mutant TCF/LEF binding sites (Ctnnb1 )
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    mutant TCF/LEF binding sites
    Available since
    March 13, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    219 Articles
    12457-plasmid-map-sequence-id-322378

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  10. pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV8)

    Viral prep
    #44362-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Publication
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Sept. 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. lenti dCAS-VP64_Blast

    Plasmid
    #61425
    Purpose
    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)
    Depositor
    Feng Zhang
    Publication
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has service
    Lentiviral Prep
    Insert
    dCAS9(D10A, N863A)-VP64_2A_Blast
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1A
    Available since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    232 Articles
    61425-plasmid-map-sequence-id-457486

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  16. hCas9

    Plasmid
    #41815
    Purpose
    Expresses human codon optimized Cas9 nuclease for genome engineering
    Depositor
    George Church
    Publication
    Mali et al Science. 2013 Feb 15;339(6121):823-6. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    human codon-optimized
    Promoter
    CMV
    Available since
    Jan. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    454 Articles
    41815-plasmid-map-sequence-id-106044

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  17. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV8)

    Viral prep
    #44361-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Publication
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Sept. 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. pCMV-PE2

    Plasmid
    #132775
    Purpose
    Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b
    Depositor
    David Liu
    Publication
    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    See manuscript
    Promoter
    CMV
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127 Articles
    132775-plasmid-map-sequence-id-259720

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  20. pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV5)

    Viral prep
    #44362-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal silencing. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Publication
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 21 - 40 of 179538 results