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Plasmid#89695Purposeto make Fosmids that can be converted into Illumina sequencable librariesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only
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EYFP-Clathrin
Plasmid#20921DepositorInsertClathrin, light polypeptide (Lca) (Clta Mouse)
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCSIIhyg-rtTA
Plasmid#139480PurposeExpresses rtTA, Tet-ON reverse tetracycline-controlled trans-activator in mammalian cells.DepositorInsertrtTA, Tet-ON reverse transactivator
UseLentiviralTagsExpressionMammalianMutationPromoterHuman EF1a promoterAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-AAVS1_sgRNA
Plasmid#183891PurposepX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.DepositorInsertAAVS1 sgRNA spacer (AAVS1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAJAK-71 (pESC-NatMXsyn-USER)
Plasmid#78232PurposeEmpty vector, for the insertion of gRNA expression cassettes into the vector.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
TR-Csy4-H29A
Plasmid#80602PurposeExpresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertCsy4-H29A
UseAAVTagsExpressionMammalianMutationHis29 mutated to AlaPromoterCBAAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-rat Nrxn2beta AS4(-)
Plasmid#58268PurposeExpression plasmid for HA-tagged rat Neurexin 2beta, lacking the insert at alternatively spliced segment 4 (AS4-)DepositorInsertNrxn2beta AS4(-) (Nrxn2 Rat)
UseTagsHAExpressionMammalianMutationPromoterCAGAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG25-L-IFP-F[1] (clon 3)
Plasmid#52899PurposePlasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.DepositorInsertL-IFP-F[1]
UseVector for homologous recombinationTagsExpressionMutationPromoterTEF promoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG32-L-IFP-F[2] (clon 3)
Plasmid#52900PurposePlasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.DepositorInsertL-IFP-F[2]
UseVector for homologous recombinationTagsExpressionMutationPromoterTEF promoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMLS791
Plasmid#188889PurposeTargeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFPDepositorInsertfloxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
UseTagsExpressionWormMutationC. elegans codom optimization and intron additionPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag FKHR H215R
Plasmid#13509DepositorInsertFKHR H215R (FOXO1 Human)
UseTagsFlagExpressionMammalianMutationH215RPromoterAvailable sinceDec. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a-R4-uAb
Plasmid#101801PurposeBacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAbDepositorInsertR4-uAb
UseTags6xHis and FlagExpressionBacterialMutationPromoterT7Available sinceNov. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
P[acman]-Alpha2
Plasmid#165859PurposeAlpha2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_8
Plasmid#60579Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_8
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-ZFP57
Plasmid#102716PurposeExpress DNA binding domain of human Zinc finger protein 57 in E. coliDepositorInsertZinc finger protein 57 (ZNF57 Human)
UseTagshisSUMOExpressionBacterialMutationPromoterT7Available sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-sfGFP-GMRWhite
Plasmid#165907PurposeBlasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_12
Plasmid#60583Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_12
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nDsRedFL-miR124
Plasmid#177806PurposeThe pre-miR-124 sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.DepositorInsertmiR-124
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMGE-T7
Plasmid#153021PurposeSingle-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-H2B-pr-mEosFP
Plasmid#141101PurposeH2B fused to pr-mEosFP for chromatin visualization and primed conversionDepositorInsertHistone 2B
UseTagspr-mEosFPExpressionMammalianMutationPromotersimian CMV IE94Available sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only