Addgene

We narrowed to 15,089 results for: LEA

Showing: 3841 - 3860 of 15089 results

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pMAL-his-LbCpf1-EC

Plasmid

#79008

Purpose

Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

LbCpf1

Use

Tags

MBP-6xHis-NLS

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pSLS.491

Plasmid

#184956

Purpose

Test effect of a1/a2 length on editing rpoB using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12

Use

Tags

Expression

Bacterial

Mutation

rpoB donor T1534C

Promoter

T7/lac

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMJ841

Plasmid

#39318

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A) and Histidine 840 …

Promoter

T7

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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mEmerald-Vinculin-N-21

Plasmid

#54304

Purpose

Localization: Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Vinculin (VCL Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Cas12j-8-crRNA

Plasmid

#194967

Purpose

The plasmid expresses Cas12j-8-crRNA expression elements.

Depositor

Yongming Wang

Article

Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-PGRMC2

Plasmid

#173468

Purpose

Human PGRMC2 containing an N-terminal FLAG tag

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC2 (PGRMC2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Nanog5P reporter

Plasmid

#16337

Depositor

Austin Cooney

Article

Gu et al Mol Cell Biol. 2005 Oct . 25(19):8507-19

Insert

Nanog promoter (Nanog Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Mouse Nanog promoter

Available since

Dec. 6, 2007

Availability

Academic Institutions and Nonprofits only

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pTRE-TIGHT-EGFP-donor fw copy

Plasmid

#22074

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Aug 13. ():.

Insert

TETO-eGFP

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pRc/RSV-m CBP-HA

Plasmid

#16701

Depositor

Richard Goodman

Article

Chrivia et al Nature. 1993 Oct 28. 365(6449):855-9.

Insert

CBP (Crebbp Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pDe-CAS9-D10A

Plasmid

#61477

Purpose

based on pDe-CAS9, encodes Cas9 D10A nickase variant

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

Cas9-D10A

Use

CRISPR

Tags

Expression

Plant

Mutation

catalytic Asp10 changed to Ala

Promoter

PcUbi4-2

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUDP293

Plasmid

#204228

Purpose

Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA

Depositor

Jean-Marc Daran

Article

Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

Inserts

Eubacterium rectale cas12a (Ercas12a)
BsaI_GFP drop-out_BsaI

Use

Synthetic Biology

Tags

NLS

Expression

Mutation

codon optimized for S. cerevisiae

Promoter

Saccharomyces cerevisiae PGK1 and Saccharomyces c…

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWF

Plasmid

#112899

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mWasabi.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-Ets2

Plasmid

#28128

Depositor

Pumin Zhang

Article

Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

Insert

Ets2 (ETS2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pCAG-ERT2-PhoCl-Gal4VP16-PhoCl-ERT2

Plasmid

#87693

Purpose

Express ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

ERT2-PhoCl-Gal4VP16-PhoCl-ERT2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2017

Availability

Academic Institutions and Nonprofits only

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pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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FUGW-VGLUT2-pHluorin

Plasmid

#207469

Purpose

Lentiviral expression of VGLUT2-pHluorin

Depositor

Robert Edwards

Article

Silm et al Neuron. 2019 May 22;102(4):786-800.e5. d

Insert

VGLUT2 (Slc17a6 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_HA1mut

Plasmid

#190475

Purpose

Express the histamine-insensitive control sensor GRAB_HA1mut in neurons

Depositor

Yulong Li

Article

Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

Insert

Histamine-insensitive sensor GRAB_HA1mut (control sensor)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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HA-GFP1-10-pDEST-C

Plasmid

#118369

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-GFP1-10

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-NBid-PhoCl2c-CBid

Plasmid

#164051

Purpose

Optogenetic manipulation of cell apoptosis using PhoCl2c

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NBid-PhoCl2c-CBid

Use

Tags

kozak sequence

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-mCherry-4A/4B-CAAX

Plasmid

#87699

Purpose

Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

mCherry-4A/4B-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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