Addgene

We narrowed to 12,006 results for: lic

Showing: 3801 - 3820 of 12006 results

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pREDusk-MCS

Plasmid

#188970

Purpose

For red light-repressed bacterial expression of gene of interest

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NODAL

Plasmid

#115638

Purpose

For targeted integration and inducible expression of human NODAL using doxycycline

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pFUS_A3B

Plasmid

#43852

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Use

Tags

Expression

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pFUS_A4A

Plasmid

#43853

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Use

Tags

Expression

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pAT28-proD/SaEGFP

Plasmid

#120321

Purpose

Bacterial eGFP labeling

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

eGFP with Staphylococcus aureus codon optimization

Use

Tags

Expression

Bacterial

Mutation

Promoter

proD

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pKL11-GCaMP6f bb100

Plasmid

#158983

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f

Use

Tags

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833

Promoter

Bba_J23100 (sigma 70)

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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ACE16-NLS

Plasmid

#210727

Purpose

NIR light-activated intein-based gene expression system (C-part)

Depositor

Vladislav Verkhusha

Article

Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

Insert

ACE16-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-T7-Top-T

Plasmid

#87309

Purpose

Expresses the "Top" transcript of the Split-Broccoli system under the control of the T7 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system "Top" transcript

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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phGR-SB

Plasmid

#133409

Purpose

overexpression of a human glucocorticoid receptor in mammalian cells

Depositor

Andrew Cato

Article

Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

Insert

NR3C1 (NR3C1 Human)

Use

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19-T7-Bottom-T

Plasmid

#87310

Purpose

Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the T7 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system "Bottom" transcript

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pREP

Plasmid

#51491

Purpose

pMDC32 with Rep/RepA coding sequence from the mild bean yellow dwarf virus

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Insert

Rep/RepA

Use

Plant expression t-dna

Tags

Expression

Mutation

none

Promoter

2x35S

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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HHM-iATPSnFR1.1

Plasmid

#102547

Purpose

expresses ATP sensor in bacteria

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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PAJ698

Plasmid

#178947

Purpose

Expresses P2 tail fiber (gpH) in fusion with S16 tail fiber (gp37)

Depositor

Alfonso Jaramillo

Article

Cunliffe et al ACS Synth Biol. 2022 Oct 21;11(10):3207-

Insert

Chimeric Tail Fiber Fusion P2-gpH and S16 gp37

Use

Unspecified

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDR-GW AT1.03R122K/R126K

Plasmid

#28005

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

AT1.03R122K/R126K

Use

Tags

mVenus and mseCFP

Expression

Yeast

Mutation

R122K, R126K

Promoter

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
p5E-ESyn1

Plasmid

#80804

Purpose

hSyn1 promoter fused to CMVmin

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p5E-ESyn1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pACYC.Taq.1Amb

Plasmid

#102794

Purpose

Expresses Taq DNA polymerases with a premature stop codon

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Taq DNA polymerase

Use

Tags

Expression

Bacterial

Mutation

W168Stop; *(see below)

Promoter

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAX1

Plasmid

#117397

Purpose

allelic exchange vector with GFP merodiploid tracker and temperature-sensitive origin of replication

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGL0_12 [pAMβ1 ORI]

Plasmid

#198926

Purpose

Level 0 part

Depositor

Cultivarium Tools, Nili Ostrov, Charlie Gilbert

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pAMβ1 ORI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pME-tdTomato no stop

Plasmid

#82404

Purpose

Gateway-compatible tdTomato fusion reporter

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

tdTomato (lacks stop codon)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pJet1.2 Tev-Avi-tag

Plasmid

#117128

Purpose

C-terminal Tev-Avi tag Tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Tev Avi-tag

Use

Golden gate donor vector for gene targeting in ba…

Tags

Tev Avi-Tag

Expression

Mutation

Promoter

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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