Showing: 361 - 380 of 921 results
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Plasmid#128166PurposeMonomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, unskippable linker)DepositorInsertmTagBFP2-T2A-sfGFP-iCre-ERT2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSCGB3A2_HL-P2A-TagBFP-PGK-NeoR-SCGB3A2_HR
Plasmid#126697Purposedonor vector for targeting a 2A peptide followed by blue fluorescent reporter to the human SCGB3A2 locus at the end of the endogenous coding sequenceDepositorInsertTagBFP
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
JDW 832 (pDONR221-V5-mTagBFP2-KRAS4A-G12D)
Plasmid#156404PurposeGateway middle entry clone encoding V5-tagged mTagBFP2-fused to human KRAS4A with G12D mutation, includes a stop codonDepositorInserthuman mutant KRAS4A-G12D (KRAS Human)
UseGateway cloningTagsmTagBFP2ExpressionMutationmutant KRAS4A-G12DPromoterAvailabilityAcademic Institutions and Nonprofits only -
JDW 827 (pCS2-V5-mTagBFP2-hsKRAS4A-G12D)
Plasmid#156409PurposepCS2 CMV expression vector driving V5-tagged mTagBFP2 fused human KRAS4A-G12DDepositorInserthuman mutant KRAS4A-G12D (KRAS Human)
UseTagsmTagBFP2ExpressionMammalianMutationmutant KRAS4A-G12DPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP
Plasmid#171090PurposeCRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.DepositorInsertsSth1 dCas9
Chloramphenicol acetyltransferase
TagBFP
UseCRISPR; Toxoplasma gondii expressionTags3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…ExpressionMutationD9A, H599APromoterTUB1 (Toxoplasma gondii), transcriptionally linke…AvailabilityAcademic Institutions and Nonprofits only -
PHY98-LMNA 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)
Plasmid#164046PurposeCRISPR donor plasmid to insert TriTag (mTagBFP) into the N-terminus of human LMNA geneDepositorInsertmTagBFG-TriTag
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR
Plasmid#187089PurposeGlutamate sensor for localization to membrane contatctDepositorArticleInsertPre-SPHERE-SF-iGluSnFR
UseTagsTagBFP-P2AExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TagBFP-P2A-Pre-SPHERE-iGABASnFR
Plasmid#187091PurposeGABA sensor for localization to membrane contatctDepositorArticleInsertPre-SPHERE-iGABASnFR
UseTagsTagBFP-P2AExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin
Plasmid#187952PurposeFKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertFKBP12_F36V-CasRx-tagBFP
UseCRISPR and Synthetic BiologyTagsGGS linker, HA-tag, and NLSExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-Vav2cat
Plasmid#178870PurposeMammalian expression of Vav2(DH-PH-CRD) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HADepositorInsertPB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-Vav2(DH-PH-CRD) (Vav2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-SOScat
Plasmid#178871PurposeMammalian expression of SOS1(Rem-Cdc25) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HADepositorInsertPB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-SOS1(Rem-Cdc25) (SOS1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA
Plasmid#193968PurposeEncodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by CAG promoterDepositorInserthPol eta (POLH Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-puro-TagBFP-β-ACTIN-3′UTR-8xMS2
Plasmid#205160Purposeexpress β-ACTIN-3′UTR-8xMS2DepositorInsertβ-ACTIN-3′UTR-24xMS2
UseTagstagBFPExpressionBacterial and MammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2
Plasmid#205161Purposeexpress β-ACTIN-3′UTR-24xMS2DepositorInsertβ-ACTIN-3′UTR-24xMS2
UseTagstagBFPExpressionBacterial and MammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCA19-HSPB1 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)
Plasmid#199450PurposeCRISPR donor plasmid to insert TriTag (mTagBFP harbors 12XMS2 in its intron) into the C-terminus of human HSPB1 geneDepositorInsertHDR donor template
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCA22-SEC61B 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)
Plasmid#199453PurposeCRISPR donor plasmid to insert TriTag (mTagBFP) into the C-terminus of human SEC61B geneDepositorInsertHDR donor template
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro
Plasmid#211462PurposeLentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLIDepositorInsertApaLI
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterTREAvailabilityAcademic Institutions and Nonprofits only -
TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro
Plasmid#211463PurposeLentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)DepositorInsertApaLI-dead
UseLentiviralTagsFLAGExpressionMammalianMutationD211A, E225A, K227APromoterTREAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2- GABARAP
Plasmid#212106PurposeExpression of GABARAP split tandem protein with mTagBFP2DepositorInsertGABARAP(G116A)-mTagBFP2-GABARAP (GABARAP Human, Entacmaea quadricolor)
UseTagsmTagBFP2ExpressionMammalianMutationG116A (lipidation deficiency) of upstream GABARAPPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GABARAP(5X/G116A)-mTagBFP2-GABARAP
Plasmid#212107PurposeExpression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23ADepositorInsertGABARAP(5X/G116A)-mTagBFP2-GABARAP (GABARAP Human, Entacmaea quadricolor)
UseTagsmTagBFP2ExpressionMammalianMutation5X: K2A, K13A, R15A, K20A, K23A mutations of upst…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 361 - 380 of 921 results