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FUW-tetO-SOX9

Plasmid

#141404

Purpose

Inducible Expression of SOX9

Depositor

Lorenz Studer

Article

Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

Insert

SOX9 (SOX9 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-puro

Plasmid

#45561

Depositor

Michael McVoy

Article

Abbate et al Biotechniques. 2001 Aug;31(2):336-40.

Insert

Puromycin N-acetyl transferase

Use

Bifunctional selection protein

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Phcmv (Strong human cytomegalovirus immediate ear…

Available since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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PKA-KTR-mStrawberry reporter

Plasmid

#158688

Purpose

This plasmid is a PKA pathway reporter. It has CMV promoter driving the expression of PKA-KTR fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode

Plasmid

#170255

Purpose

Barcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

SV40 Large T Antigen

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEF-GFP

Plasmid

#11154

Purpose

Mammalian expression vector for expression of GFP (EF1a promoter)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

EF1 alpha promoter (EEF1A1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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human p53-(1-393)

Plasmid

#24859

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Full length.

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pYPQ230-D156R (ttLbCas12a)

Plasmid

#195366

Purpose

A temperature-tolerant LbCas12a mutant (D156R), also known as ttLbCas12a

Depositor

Yiping Qi

Article

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

LbCas12a-D156R (ttLbCas12a)

Use

CRISPR; Gateway compatible lbcas12a-d156r (ttlbca…

Tags

Expression

Plant

Mutation

Promoter

ZmUBI1

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAC-SP6

Plasmid

#102418

Purpose

Ac transposase with SP6 promoter for in vitro transcription

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

Ac transposase

Use

Tags

Expression

Bacterial

Mutation

Promoter

SP6

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDisplay-BirA-ER

Plasmid

#20856

Depositor

Alice Ting

Article

Howarth et al Nat Methods. 2008 May . 5(5):397-9.

Insert

BirA-ER

Use

Tags

Expression

Mammalian

Mutation

C-terminal ER targeting sequence (KDEL)

Promoter

Available since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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pMK286 (mAID-NeoR)

Plasmid

#72824

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Neo

Use

CRISPR

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCRIS-PITChv2-Puro-dTAG (BRD4)

Plasmid

#91793

Purpose

PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

Puro-P2A-2xHA-FKBP_F36V

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

Promotorless

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-24xGCN4_v4-kif18b-24xPP7

Plasmid

#74928

Purpose

Translation reporter

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

24xGCN4_v4-kif18b-24xPP7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-1_Ec_rpoZ

Plasmid

#128837

Purpose

E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

Depositor

Seth Darst

Article

Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

Insert

rpoZ (rpoZ E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCT-tRNA

Plasmid

#133813

Purpose

CRISPR-Cas9 system for genetic manipulation of Candida tropicalis

Depositor

Geraldine Butler

Article

Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

Insert

cassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a

Plasmid

#113431

Purpose

Expresses 10xHis-MBP fused to LbCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Has Service

Cloning Grade DNA

Insert

LbCas12a

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCRIS-PITChv2-BSD-dTAG (BRD4)

Plasmid

#91792

Purpose

PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

BSD-P2A-2xHA-FKBP_F36V

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

Promoterless

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-EEA1 wt

Plasmid

#42307

Depositor

Silvia Corvera

Article

Lawe et al J Biol Chem. 2000 Feb 4;275(5):3699-705.

Insert

EEA1 (EEA1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pHIV-EGFP

Plasmid

#21373

Purpose

self-inactivating lentiviral plasmid for co-expression of your gene of interest and EGFP

Depositor

Zena Werb, Bryan Welm

Article

Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-UBR5

Plasmid

#52050

Purpose

CMV driven mammalian expression of UBR5 with an N-termianl GFP fusion

Depositor

Darren Saunders

Article

Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

Insert

UBR5 (UBR5 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-Cre-blast

Plasmid

#179390

Purpose

Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

Insert

mCherry (P2A) Cre recombinase

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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