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pEN_TT 3xHA-MKK6-EE

Plasmid

#47544

Purpose

Gateway entry vector for an inducible 3xHA-tagged constitutively active MKK6 mutant

Depositor

Kevin Janes

Article

Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

Insert

3xHA-MKK6-EE (MAP2K6 Human)

Use

Entry vector

Tags

3XHA

Expression

Mutation

S207E, T211E

Promoter

Available since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pENTR-FLAG-HAND1-T2A-mTagBFP2

Plasmid

#223192

Purpose

Entry vector containing FLAG-HAND1 with a T2A-mTagBFP2 reporter (attL flanked)

Depositor

Matthew Birket

Article

Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

Insert

HAND1 (HAND1 Human)

Use

Promoterless entry vector for gateway cloning

Tags

FLAG and T2A-mTagBFP2

Expression

Bacterial

Mutation

Promoter

None

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR1A 2xHA GPR153

Plasmid

#226808

Purpose

Gateway entry vector for 2xHA GPR153

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

GPR153 (GPR153 Human)

Use

Gateway entry

Tags

2xHA tag followed by a GDPPVAT linker

Expression

Mutation

Sequence optimized from NP_997253.2

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR1A 2xHA LTB4R

Plasmid

#226806

Purpose

Gateway entry vector for 2xHA LTB4R

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

LTB4R (LTB4R Human)

Use

Gateway entry

Tags

2xHA tag followed by a GDPPVAT linker

Expression

Mutation

Sequence optimized from NP_858043.1

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pKM 1995

Plasmid

#217826

Purpose

Expresses 1554 bp fragment of HY4 gene in A. thaliana. This HY4 fragment shows high homology to the phr genes in prokaryotes.

Depositor

Aziz Sancar

Article

Malhotra et al Biochemistry. 1995 May 23;34(20):6892-9.

Insert

HY4 (CRY1 Mustard Weed)

Use

Tags

Maltose-binding protein

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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Cav2.2 W391A pcDNA3

Plasmid

#206085

Purpose

expression of rabbit Cav2.2 calcium channel with a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

Depositor

Annette Dolphin

Article

Leroy et al J Neurosci. 2005 Jul 27;25(30):6984-96.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT mCh-V5-NES-EGFP-ERBB2 NLS

Plasmid

#192316

Purpose

Gateway entry vector for an inducible mCh-V5-NES-EGFP-ERBB2 NLS

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-ERBB2 NLS (ERBB2 Human)

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-HsNRF2 (NFE2L2)

Plasmid

#194302

Purpose

Multisite gateway entry clone for expression of human NRF2 (NFE2L2) with fusion tag at the N-terminus. Parton lab clone KRS

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

NFE2L2 (NFE2L2 Human)

Use

Multisite gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pME-MmCavin4

Plasmid

#170528

Purpose

Entry clone for tagging MmCavin4 at the C terminus. Parton lab clone ADM

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Cavin4 (Cavin4 Mouse)

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pME-MmCav1

Plasmid

#170527

Purpose

Entry clone for tagging MmCav1 at the C terminus. Parton lab clone BCJ

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Caveolin1 (Cav1 Mouse)

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ288 (BoCas12a)

Plasmid

#138125

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BoCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

BoCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ285 (Lb5Cas12a)

Plasmid

#138120

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Lb5Cas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

Lb5Cas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ286 (CMaCas12a)

Plasmid

#138122

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized CMaCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

CMaCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ233-STU

Plasmid

#138111

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated LbCas12a-SRDX repressor without promoter and terminator

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

dLbCas12a-SRDX

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ239-STU

Plasmid

#138112

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized FnCas12a without promoter and terminator

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

FnCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS-Ala271

Plasmid

#158631

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway entry vector

Tags

3XFLAG

Expression

Mutation

Q271A please see depositor comments below

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo-NCOA7g3 (BB36)

Plasmid

#139454

Purpose

Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a / U6

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo-NCOA7g2 (BB35)

Plasmid

#139453

Purpose

Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a / U6

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS-Gln93,Ala271

Plasmid

#158632

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway entry vector

Tags

3XFLAG

Expression

Mutation

E93Q/Q271A

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS-Ala148,Ala271

Plasmid

#158633

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway entry vector

Tags

3XFLAG

Expression

Mutation

Q148A/Q271A

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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