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Showing: 3761 - 3780 of 19013 results

  1. pET28-eIF4E-TwStrep

    Plasmid
    #208638
    Purpose
    for E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)
    Depositor
    Quanfu Ma
    Insert
    human eIF4E (ENST00000450253) (EIF4E Human)
    Use
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208638-plasmid-map-sequence-id-415918

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  2. pUC-T7-J1~398-Luc

    Plasmid
    #208639
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19
    Depositor
    Quanfu Ma
    Insert
    Hepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
    Use
    Luciferase; In vitro transcription template
    Tags
    Firefly Luciferase (F-Luc and T7 promoter
    Expression
    Mutation
    nt 1~398, in frame with donstream F-Luc
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208639-plasmid-map-sequence-id-418341

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  3. pET28-mEGFP(A206K)-TwStrep

    Plasmid
    #208622
    Purpose
    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone
    Depositor
    Quanfu Ma
    Insert
    mEGFP(A206K)
    Use
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    (compared with pEGFP-C1, protein level) A206K
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208622-plasmid-map-sequence-id-415916

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  4. qTAG-C-V5-Blast

    Plasmid
    #207719
    Purpose
    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Blast cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207719-plasmid-map-sequence-id-413809

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  5. qTAG-N-Zeo-V5

    Plasmid
    #207708
    Purpose
    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-V5 cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207708-plasmid-map-sequence-id-413799

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  6. qTAG-N-Blast-V5

    Plasmid
    #207686
    Purpose
    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-V5 cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207686-plasmid-map-sequence-id-413750

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  7. qTAG-N-Puro-V5

    Plasmid
    #207697
    Purpose
    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-V5 cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207697-plasmid-map-sequence-id-413759

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  10. pYTK001_P2Ab

    Plasmid
    #183084
    Purpose
    2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly
    Depositor
    Zhen Wang
    Article
    Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.
    Insert
    P2A
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    183084-plasmid-map-sequence-id-367243

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  13. pKP1627

    Plasmid
    #167648
    Purpose
    Express FepA G54C, delta12-16 (TonB box) . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    fepA G54C, delta12-16 (TonB box)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    fepA with G54C, delta12-16 (TonB box) mutations (…
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167648-plasmid-map-sequence-id-339798

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  15. pKP1626

    Plasmid
    #167646
    Purpose
    Express FepA D34C, delta12-16 (TonB box) . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    fepA D34C, delta12-16 (TonB box)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    fepA with D34C, delta12-16 (TonB box) mutations. …
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167646-plasmid-map-sequence-id-335414

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Showing: 3761 - 3780 of 19013 results