pHMGWA-Cas1
(Plasmid
#122285)
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PurposeCas1 cloned in pHMGWA
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122285 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepHMGWA
- Backbone size w/o insert (bp) 6626
- Total vector size (bp) 7544
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameEco Cas1
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Insert Size (bp)918
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHMGWA-Cas1 was a gift from Jennifer Doudna (Addgene plasmid # 122285 ; http://n2t.net/addgene:122285 ; RRID:Addgene_122285) -
For your References section:
Cas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity. Nunez JK, Kranzusch PJ, Noeske J, Wright AV, Davies CW, Doudna JA. Nat Struct Mol Biol. 2014 Jun;21(6):528-34. doi: 10.1038/nsmb.2820. Epub 2014 May 4. 10.1038/nsmb.2820 PubMed 24793649
Map uploaded by the depositor.
