Addgene

We narrowed to 23,006 results for: GFP

Showing: 3701 - 3720 of 23006 results

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FCK-ChrimsonR-GFP

Plasmid

#59049

Purpose

Lentiviral expression of ChrimsonR under the CaMKII promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

K176R

Promoter

CaMKII

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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6K-loop-->R GLUT1-GFP

Plasmid

#200098

Purpose

human GLUT1 with K-->R mutation of 6 lysines in the major cytoplasmic loop and C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 225, 229, 230, …

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAT15516_BEAR-GFP-2in1

Plasmid

#174082

Purpose

BEAR-GFP plasmid with a gRNA that targets its own plasmid

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Tags

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pF144 pAcGFP1 SOD1G85R

Plasmid

#26405

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

G85R

Promoter

Available since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only
5K-tails-->R GLUT1-GFP

Plasmid

#200099

Purpose

human GLUT1 with K-->R mutation of 5 lysines on the N- and C-terminal tails with a C-terminal tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 6, 7, 451, 456,…

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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1K245 GLUT1-GFP

Plasmid

#200097

Purpose

human GLUT1 with a single cytosolic lysine (K245) and C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 6, 7, 225, 229,…

Promoter

CMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pJH625_pJH_LldR_PlldR-sfGFP.0TAG

Plasmid

#163724

Purpose

Lactate-inducible expression of green fluorescent protein encoding zero amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-sfGFP-DsRed

Plasmid

#80811

Purpose

Vector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Kate O'Connor-Giles

Insert

sfGFP-piggyBac-DsRed

Use

Unspecified

Tags

sfGFP

Expression

Mutation

Promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pFX-β-tubulin-EGFP

Plasmid

#175827

Purpose

Express EGFP with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with microtuble-targeting sequence

Use

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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1GFP/RNase H1 D210N

Plasmid

#174448

Purpose

Express GFP-tagged RNase H1 D210N in E. coli.

Depositor

Karlene Cimprich

Article

Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

RNase H1 (RNASEH1 Human)

Use

Tags

His-tag, GFP

Expression

Bacterial

Mutation

D210N

Promoter

T7 promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-egfp-U3-Osp.gRNA

Plasmid

#166034

Purpose

Empty backbone for expression of gRNA-expressing integration deficient lentiviral particles.

Depositor

Yujia Cai

Article

Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-15F11-HA-mEGFP

Plasmid

#129590

Purpose

The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Article

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pGEMHE-membrane-mEGFP

Plasmid

#105526

Purpose

T7 promotor drives in vitro mRNA transcription of a mEGFP-tagged membrane reporter

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

GNAI2 (GNAI2 Human)

Use

In vitro transcription

Tags

mEGFP

Expression

Mutation

fragment encoding the plasma membrane targeting s…

Promoter

T7

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/V5-DEST-GFP

Plasmid

#40125

Depositor

Lynda Chin

Article

Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

Insert

GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Flag-humanRBM47

Plasmid

#134607

Purpose

Plasmid bearing RBM47 coding sequence. Flag tagged is useful for IP and WB assays.

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

Flag-humanRBM47 (RBM47 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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FoxO1-ADA-GFP

Plasmid

#35640

Depositor

Domenico Accili

Article

Qiang et al J Biol Chem. 2010 Aug 27;285(35):27396-4

Insert

FoxO1-ADA-GFP (Foxo1 Mouse)

Use

Tags

Flag, GFP, and Myc

Expression

Mammalian

Mutation

T24A, S253D and S316A mutations

Promoter

CMV

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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FCK-ChR2-GFP

Plasmid

#15814

Purpose

Lentiviral expression of ChR2-GFP for neuronal excitation

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

ChR2

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CaMKII

Available since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pCD5-H5_Vietnam_1203/04_sfGFP_ST

Plasmid

#182546

Purpose

Expresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virus

Depositor

Robert de Vries

Article

Broszeit et al Cell Rep. 2019 Jun 11;27(11):3284-3294.e

Insert

H5_Vietnam_1203_04

Use

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti6-DCX-EGFP

Plasmid

#80599

Purpose

pLenti6 destination vector encoding C-terminally EGFP-tagged human doublecortin (DCX; GenBank: NM_178152)

Depositor

Torsten Wittmann

Article

Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. d

Insert

Doublecortin (DCX Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

CMV

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

Plasmid

#174892

Purpose

amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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