-
Plasmid#199725PurposeA pVS1-based T-DNA binary vector and destination vector for Gateway cloning with NPTII as a plant selectable marker gene.DepositorInsertattR1/R2 cassette
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCB-CBEv1
Plasmid#221138PurposeCoxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.DepositorInsert3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
UseCRISPRTags3xFLAGExpressionBacterialMutationPromoterlacIq-PtacAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
3TERM_35S
Plasmid#136114Purpose3TERM after CDS/ CDS12-CTAGDepositorInsert3TERM_35S (CaMV)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL2_169
Plasmid#199101PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpIJ101 ORI plus NGS barcode 15, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISYmu1_reRNA
Plasmid#205358PurposeBacterial expression of ISYmu1 TnpB and its reRNADepositorInsertISYmu1 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pylT-AznLRS
Plasmid#172482PurposeFor E.Coli expression, 306L309L348I384F411WDepositorInsertAznLRS and pylTcua
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
FU-tetO-Gateway
Plasmid#43914DepositorTypeEmpty backboneUseLentiviral; Gateway destination vector, doxycycli…Tags6xHis and V5ExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAureus-TnCAM
Plasmid#199807Purposevector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureusDepositorInserttransposon cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB109
Plasmid#68563PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTags3xFLAGExpressionPlantMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG- gate-pGK-HYG
Plasmid#107397PurposeGateway destination vectorDepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWJ66
Plasmid#46570PurposeSame as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)DepositorInsertstracrRNA
dcas9-w
CRISPR array
UseCRISPRTagsrpoZExpressionBacterialMutationD10A, H840APromoterAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR-221-P3-P4
Plasmid#186351PurposeGateway donor vector with AttP3 AttP4 recombination sitesDepositorTypeEmpty backboneUseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHEA
Plasmid#168297PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
UseTags6xHis, E-tag, and PelB signal peptideExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEMPTY::sgRNA2
Plasmid#165459PurposeEscherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteriaDepositorInsertsgRNA2
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterSP01Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMVS1111A:PhmtB-bgaB
Plasmid#205998PurposeConjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus encoding beta-galactosidaseDepositorInsertthermostable beta-galactosidase
UseTagsExpressionMutationPromoterPhmtBAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MbAcK3RS(IPYE)
Plasmid#104070PurposeExpression plasmid encoding MbAcK3RS(IPYE) and pylTDepositorInsertPlpp MbAcK3RS(IPYE); PproK pyl
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti_C_Myc_DDK_IRES_Puro_PRKRA
Plasmid#106113PurposeLentiviral expression of PRKRADepositorInsertPRKRA (PRKRA Human)
UseLentiviralTagsDDK (FLAG) and MycExpressionMutationPromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
CDS_mALS
Plasmid#136081PurposeConfers plant resistance to chlorsulfuronDepositorInsertCDS_mALS
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRU55
Plasmid#167684PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::asCpf1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MmPylRS(IPYE)
Plasmid#104073PurposeExpression plasmid encoding MmPylRS(IPYE) and pylTDepositorInsertPlpp MmPylRS(IPYE); PproK pyl
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only