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Plasmid#172488PurposeBacterial expression of codon-optimized His-SUMO-LbuCas13a.DepositorInsertbdSUMO-LbuCas13a (codon-optimized)
UseTagsHis6 and bdSUMOExpressionBacterialMutationPromoterT7 promoter and lac operatorAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-dnMCAK
Plasmid#208650PurposeMammalian expression plasmid expressing eGFP-dnMCAK driven by CMV promoterDepositorInsertKif2c (Kif2c Cricetulus griseus (Chinese hamster))
UseTagseGFPExpressionMammalianMutationS92A , S106A , S108A, S112A , S186A, H530A, R534A…PromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-FOSL2-P2A-mCherry
Plasmid#203860PurposeTet-inducible lentiviral plasmid expressing FOSL2-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertFOSL2 (FOSL2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB-Tet-On-HTR6-RhoA sensor
Plasmid#189614PurposeTet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); HTR6-fused with a sGFP-mScarlet-I based sensorDepositorInsertHTR6 (HTR6 Human)
UseTagsGFP and mScarletExpressionMammalianMutationPromoterTet3GAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-ID2-P2A-mCherry
Plasmid#203865PurposeTet-inducible lentiviral plasmid expressing ID2-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertID2 (ID2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-PdCO-EGFP
Plasmid#198512PurposeExpresses optimized PdCO in frame with EGFP under control of CMV promotor.DepositorInsertPdCO
UseTagsEGFP and Rho1D4ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR003-sgTP53-2
Plasmid#118020PurposeConstitutive expression of sgRNA targeting human TP53DepositorInsertsgTP53-2 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-GLI1-P2A-mCherry
Plasmid#203858PurposeTet-inducible lentiviral plasmid expressing GLI1-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertGLI1 (GLI1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR003-sgTP53-4
Plasmid#118022PurposeConstitutive expression of sgRNA targeting human TP53DepositorInsertsgTP53-4 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-YFP-KRAS4B
Plasmid#112718PurposeExpresses YFP-KRAS4B fusion protein used for FRET studies in eukaryotic cells.DepositorInsertYFP-KRAS4B (KRAS Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTBL227 NheI-Cas9-KpnI-5xFlag-FseI-TdT-NotI
Plasmid#126477PurposeExpresses Cas9-5xFlag-TdT in mammalian cells.DepositorInsertsUseTagsExpressionMammalianMutationChanged codons to reduce repetitive sequences.PromoterCMVAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAHR TH-p2a-TD:Tomato (floxed selection Puro)
Plasmid#135814PurposeFluorescent reporter for knock-in of TdTomato into the TH locusDepositorInsertTH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)
UseTagsp2a-TdTomatoExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-MAFB-P2A-mCherry
Plasmid#203866PurposeTet-inducible lentiviral plasmid expressing MAFB-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertMAFB (MAFB Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX_305-N-dTAG-FRA1
Plasmid#188742Purposeexpresses murine Fosl1 (Fra1) with N-terminal tag FKBP F36V (dTAG) for the dTAG systemDepositorInsertFosl1 (Fosl1 Mouse)
UseLentiviralTagsFKBP F36V tag (dTAG)ExpressionMammalianMutationPromoterhPGK promoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEx-iSeroSnFR
Plasmid#128486PurposeFluorescent reporter for serotonin (conditional + membrane localization tag)DepositorHas ServiceAAV9InsertiSeroSnFR
UseAAVTagsIg-kappa leader, Myc, PDGFR, and cpsfGFPExpressionMammalianMutationPromoterCAGAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXPR003-sgTP53-1
Plasmid#118019PurposeConstitutive expression of sgRNA targeting human TP53DepositorInsertsgTP53-1 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSITEP-puro-shWRN1-C911
Plasmid#125784Purposea seed-matched control for pRSITEP-puro-shWRN1DepositorInsertshWRN1-C911 (WRN Human)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSITEP-puro-shWRN2-C911
Plasmid#125786Purposea seed-matched control for pRSITEP-puro-shWRN2DepositorInsertshWRN2 (WRN Human)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-EGR1-P2A-mCherry
Plasmid#203856PurposeTet-inducible lentiviral plasmid expressing EGR1-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertEGR1 (EGR1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-shRFP
Plasmid#125778Purposeconstitutive expression of a short-hairpin RNA targeting RFPDepositorInsertshRFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only