Tet-O-FUW-EGR1-P2A-mCherry
(Plasmid
#203856)
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PurposeTet-inducible lentiviral plasmid expressing EGR1-P2A-mCherry, with ORF specific barcode close to 3'LTR site
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203856 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneTet-O-FUW
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth TemperatureRoom Temperature
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGR1
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SpeciesH. sapiens (human)
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Entrez GeneEGR1 (a.k.a. AT225, G0S30, KROX-24, NGFI-A, TIS8, ZIF-268, ZNF225)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Tet-O-FUW-EGR1-P2A-mCherry was a gift from Andrea Califano (Addgene plasmid # 203856 ; http://n2t.net/addgene:203856 ; RRID:Addgene_203856) -
For your References section:
Systematic elucidation and pharmacological targeting of tumor-infiltrating regulatory T cell master regulators. Obradovic A, Ager C, Turunen M, Nirschl T, Khosravi-Maharlooei M, Iuga A, Jackson CM, Yegnasubramanian S, Tomassoni L, Fernandez EC, McCann P, Rogava M, DeMarzo AM, Kochel CM, Allaf M, Bivalacqua T, Lim M, Realubit R, Karan C, Drake CG, Califano A. Cancer Cell. 2023 May 8;41(5):933-949.e11. doi: 10.1016/j.ccell.2023.04.003. Epub 2023 Apr 27. 10.1016/j.ccell.2023.04.003 PubMed 37116491
