-
Plasmid#35503PurposeAAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.DepositorInserthChR2(C128S/D156A)-EYFP
UseAAVTagsEYFPExpressionMammalianMutationC128S and D156APromoterEf1aAvailable sinceApril 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a DIO-ChRmine-oScarlet-Kv2.1-WPRE
Plasmid#183525PurposeOptogeneticsDepositorInsertChRmine-oScarlet-Kv2.1
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UAS-anti-CD3/anti-CD19 BiTE
Plasmid#230005PurposeGal4-dependent expression of secreted BiTEDepositorInsertUAS-anti-CD3/anti-CD19 BiTE
UseAAVTagsExpressionMammalianMutationPromoterUASAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry
Plasmid#212829PurposeDoxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.DepositorInsertZIM3-KRAB-dCas9-P2A-mCherry
UseTagsHA-tag and ZIM3-KRABExpressionBacterial and MammalianMutationPromoterCAG, TetOnAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV CMV-DIO-(mCherry-U6)-shRNA(anti-Crh)
Plasmid#214732PurposeExpresses shRNA against CRH, marked with mCherry, in infected and cre positive cellsDepositorInsertanti-Crh shRNA / mCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
Plasmid#92392Purposeproduces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTADepositorInsertTM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterHuman SynapsinAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP
Plasmid#111585PurposeAn AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.DepositorInsertmyriRFP670V5-P2A-post-eGRASP
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EWB-DIO-cyan pre-eGRASP(p32)
Plasmid#111589PurposeAn AAV vector that expresses double floxed cyan pre-eGRASP(p32) under the Ef1a promoter.DepositorInsertcyan pre-eGRASP(p32)
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA
Plasmid#84474PurposepAAV-RAM-NLS-mKate2DepositorInsertmKate2
UseAAVTagsNuclear localization signal of SV40 large T antig…ExpressionMutationPromoterTREAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-DR30(SapI) 0.5 Syn-intron-hfCas13d-pA
Plasmid#233039PurposeTo express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a 0.5 bp synapsin promoterDepositorInsertRfxCas13d-compatible gRNA expression cassette and hfCas13d
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP1795 - pAAV-AiE0170h-minBG-iCre(R297T)-BGHpA
Plasmid#220717PurposeAiE0170h is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cellsInsertiCre(R297T)
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
Plasmid#125228PurposeExpresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cellsDepositorInsertNanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
UseAAVTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR
Plasmid#207878PurposeAAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).DepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-GFAP-NLS-CasRx-NLS-FLAG-U6-DR-SgRNA_Ptbp1-DR
Plasmid#154001PurposeVector expressed CasRx and sgRNA_Ptbp1 for AAV packageDepositorInsertCasRx, Ptbp1 sgRNAs
UseCRISPR and Mouse TargetingTagsFLAGExpressionMutationPromoterGFAP, U6Available sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
AiP20027 - pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA
Plasmid#220735PurposeAiE2182m_3xC2 is an optimized enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cellsInsertiCre(R297T)
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP1976 - pAAV-AiE2569m-minBG-SYFP2-WPRE-BGHpA
Plasmid#214600PurposeAiE2569m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsInsertSYFP2
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pA
Plasmid#37090PurposeExpresses humanized ChR2(H134R)-mCherry in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitationDepositorInserthChr2(H134R)-mCherry
UseAAV and Cre/Lox; Cre-offTagsmCherryExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA
Plasmid#84468PurposepAAV-CRAM-tdTomatoDepositorInserttdTomato
UseAAVTagsExpressionMutationT230I (please see depositor comments below)PromoterTREAvailable sinceDec. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
(565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP
Plasmid#163030PurposeExpression of CRISPRi with gSTOP gRNADepositorInsertMammalian codon-optimized SadCas9
UseAAVTagsKRAB, SV40 pA, and myc NLSExpressionMammalianMutationPromoterAlb-AATAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
AiP1010-pAAV-mscRE4-minBGpromoter-iCre-WPRE-hGHpA
Plasmid#163476PurposeDirect-expressing iCre AAV Virus. Alias: AiP1010 - pAAV-AiE2004m-minBG-iCre-WPRE-HGHpAInsertiCre
UseAAVTagsExpressionMutationPromoterBeta Globin minimal promoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only