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Plasmid#208035PurposeEnables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycinDepositorInsertSUMO1nc (SUMO1 Human)
UseLentiviralTagsMyc, C-terminal Split-TurboIDExpressionMutationQ94P Mutation near C-terminus suppresses cleavage…PromotertetO/UBCAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-Puro-dTAG (BRD4)
Plasmid#91793PurposePITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.DepositorInsertPuro-P2A-2xHA-FKBP_F36V
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-MCS-GFP-SV-puro
Plasmid#73582PurposeThird generation lentiviral vector expressing GFP and puromycinDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsGFP tagExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-pcDNA3.1 Puro-CAG-Voltron2-ST
Plasmid#172910PurposeMammalian expression of voltage sensor; soma localization targeting sequenceDepositorInsertVoltron2-ST
UseTagssoma localization targeting sequenceExpressionMammalianMutationA122DPromoterCAGAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro mouse IRS-1 myc
Plasmid#11374DepositorInsertIRS-1 (Irs1 Mouse)
UseRetroviralTagsmycExpressionMammalianMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hOCT3/4-shp53-F + mCherry-2A-puro
Plasmid#74947PurposeDosage control and tracing of reprogramming episomesDepositorInsertOct3/4 (POU5F1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-dCas9-p300-P2A-PuroR
Plasmid#83889PurposeLentiviral Sp dCas9-p300-P2A-PuroRDepositorInsertsS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
pac from Streptomyces alboniger
UseCRISPR and LentiviralTagsFlag and nucleoplasmin NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEFSAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
lenti sgRNA(MS2)_puro backbone
Plasmid#73795Purpose3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-Halo-hMRE11(H129N)
Plasmid#208394PurposeTo generate stable cell line expressing Halo-human MRE11(H129N) by Retroviral infection.DepositorInsertHuman MRE11 (MRE11 Human)
UseLuciferase and RetroviralTagsExpressionMammalianMutationH129NPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
MMLV-CAG-TVA-IRES-Puro
Plasmid#172366PurposeGeneration of TVA-expressing stable cell lines, resistant to the antibiotic PuromycinDepositorInsertTVA + Puro
UseRetroviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIN4-EF1a-ETV2-IRES-Puro
Plasmid#61061Purposelentiviral vector for ETV2DepositorInsertETV2 (ETV2 Human)
UseLentiviralTagsExpressionMutationPromoterEF1alphaAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Puro DEST (670-1)
Plasmid#17293Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, PuroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVTRE3G eGFP Puro (w819-1)
Plasmid#27570DepositorInsertEnhance Green Fluorescent Protein
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-puro-PTPIP51(1-470)
Plasmid#170536PurposeExpresses PTPIP51(1-470) in human HeLa cells after transfectionDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterCAG promoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro mouse IRS-2 myc
Plasmid#11373DepositorInsertIRS-2 (Irs2 Mouse)
UseRetroviralTagsmycExpressionMammalianMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TetON-Puro-HIF1a 3809
Plasmid#118705PurposeLentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)DepositorInsertshRNA against human HIF1a
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK GFP Puro (w509-5)
Plasmid#19070Purpose3rd gen lentiviral eGFP expression vector, PGK promoter, PuroDepositorInsertenhanced green fluorescent protein
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDRM209 LTP (Luciferase tdTomato Puro)
Plasmid#174723PurposeExpresses the firefly luciferase gene, E2A, tdTomato, T2A, and the puromycin resistance gene.DepositorInsertsFirefly Luciferase
tdTomato
pac
UseLentiviral and LuciferaseTagsE2A linker and T2A linkerExpressionMammalianMutationPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-TERT-IRES-Puro
Plasmid#182590Purposeexpresses TERT in mammalian cellsDepositorInserthTERT (TERT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-pcDNA3.1 Puro-CAG-Voltron2
Plasmid#172909PurposeMammalian expression of voltage sensorDepositorInsertVoltron2
UseTagsExpressionMammalianMutationA122DPromoterCAGAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only