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FSW-ALDH2-E504K-T2A-EGFP

Plasmid

#197037

Purpose

Express ALDH2 with E504K mutation from hSyn1 promoter, co-expressed with EGFP

Depositor

Ronald Hart

Article

Sun et al Aging Cell. 2023 Feb;22(2):e13772. doi:

Insert

ALDH2-E504K

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

E504K

Promoter

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+N-eGFP_SAMD9L_R986C

Plasmid

#206176

Purpose

mammalian expression of SAMD9L R986C

Depositor

Christopher Goodnow

Article

Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

Insert

SAMD9L (SAMD9L Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

R986C

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pSR-puro-Mff1 shRNA

Plasmid

#37247

Depositor

David Kashatus, Christopher Counter

Article

Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

Insert

Mff1 (MFF Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pRS416-Aga1p-FAPB2.3.6L1TAG

Plasmid

#158148

Purpose

Yeast display reporter for non-display yeast strains (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

Aga1p-FAPB2.3.6

Use

Tags

Expression

Yeast

Mutation

Cloned in a TAG codon at the first position of th…

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCSDest-MYC-TMPRSS2

Plasmid

#154964

Purpose

TMPRSS2 with N-terminal MYC

Depositor

Utpal Pajvani

Article

Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

Insert

MYC-TMPRSS2 (TMPRSS2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCfB3035(X-4 MarkerFree)

Plasmid

#73273

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pClgn1.1-Trim41-PA-1D4

Plasmid

#186339

Purpose

Express Trim41-PA-1D4 under the mouse Clgn promoter.

Depositor

Masahito Ikawa

Article

Oura et al PLoS Genet. 2022 Jun 1;18(6):e1010241. d

Insert

Trim41 (Trim41 Mouse)

Use

Tags

1D4 and PA

Expression

Mammalian

Mutation

Promoter

pClgn1.1

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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mRIP3 GFP D161N

Plasmid

#41383

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (Ripk3 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Kinase Domain Mutant (D161N)

Promoter

CMV

Available since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CMV-CIBN-mCherry-VAMP2

Plasmid

#178573

Purpose

Mammalian expression plasmid of CIBN-VAMP2 of Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CIBN-mCherry-VAMP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB3034(X-3 MarkerFree)

Plasmid

#73272

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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p1977 (pUC19-PTDH3-PTEF1)

Plasmid

#78230

Purpose

Example plasmid containing a bidirectional promoter of pTDH3 and pTEF1

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

Bidirectional promoters pTDH3-pTEF1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
mH2A2-CT-MYC

Plasmid

#45170

Depositor

Hunt Willard, Brian Chadwick

Article

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy2 (MACROH2A2, H2AFY2 Human)

Use

Tags

HIS and MYC

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pNIC-Bio2_SMAC(delta(55))_9His

Plasmid

#197976

Purpose

E. coli expression of SMAC(delta(55))-9His

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

Smac (DIABLO Human)

Use

Tags

9His

Expression

Bacterial

Mutation

delta (1-55)

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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tdEos-VSVG-16

Plasmid

#57693

Purpose

Localization: Golgi/ER Transport, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

VSVG

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5/FRT/TO V5 DNAJB4

Plasmid

#19526

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DnaJ (Hsp40) homolog, subfamily B, member 4 (DNAJB4 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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Flag-Craf S338A

Plasmid

#118335

Purpose

Expression of human Craf

Depositor

Philip Stork

Article

Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

Insert

Craf S338A (RAF1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S338A

Promoter

CMV

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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HA-Cdk9 T186A

Plasmid

#28103

Depositor

Andrew Rice

Article

Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

Insert

Cdk9 (CDK9 Human)

Use

Tags

3x HA

Expression

Bacterial and Mammalian

Mutation

Contains T186A mutation in Cdk9

Promoter

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (Frag E)

Plasmid

#16609

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Frag E) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

FragE (SmaI->XhoI)

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pCTCON2-GFP-TAG

Plasmid

#158131

Purpose

Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

sfGFP

Use

Tags

Expression

Yeast

Mutation

Introduced a stop codon in place of the tyrosine …

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pUSE-Src-KD-UniRapR-mCerulean-myc

Plasmid

#45382

Depositor

Klaus Hahn

Article

Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

Src-KD-UniRapR-mCerulean-myc

Use

Tags

mCerulean and myc

Expression

Mammalian

Mutation

mSrc R388D (kinase dead), Y529F

Promoter

CMV

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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