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Plasmid#122659PurposeExpresses sgRNA for telomere repeats with fluorescent indicator BFP and puromycin selection markerDepositorInsertBFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-gRNA-puro
Plasmid#180426PurposeExpresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.DepositorInsertgRNAscr
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
attB53-Pac-attB53
Plasmid#183610PurposeRMCE vector to shuttle puromycin resistance into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).DepositorInsertPac
UseRmceTagsExpressionMutationPromoterAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-mKate2-3xNLS-IRES-Pac
Plasmid#183618PurposeMammalian expression vector for mKate2-3xNLS from the CAG promoter. Confers puromycin resistance.DepositorInsertmKate2-3xNLS
UseTags3xNLSExpressionMammalianMutationPromoterCAGAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
TOPO PuroR
Plasmid#68447PurposeTOPO construct expressing PuroR for generation of GMAP-compatible geneDepositorInsertPuromycin Resistance
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
betaRBPIG
Plasmid#16422DepositorInsertloxP flanked RFP and blasticidin-resistance plus puromycin-acetyltransferase IRES GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE2-puro
Plasmid#208032PurposeEnables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-9A
Plasmid#114023PurposeInducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell linesDepositorInserteGFP-HEC1 - 9A (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 9APromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3PA-Puro-iTk
Plasmid#109067Purposeinserts three polyA sites to end transcription of a targetted gene and a puromycin cassette for selectionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEFSAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
Myog'-2A-mCherryNLS-PGK-Purodtk
Plasmid#69547PurposeDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.DepositorInsertsUseMouse TargetingTags2x nuclear localization signal and PVT1-2A "…ExpressionMutationSilent V205V mutation in myogenin gene to prevent…PromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTS1021-Tier1-PhCMV-PuroR
Plasmid#169492PurposeTier-1 vector encoding PhCMV-driven marker for puromycin selection (PhCMV-PuroR-pA)DepositorInsertPCMV-driven puromycin N-acetyl-transferase
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-sg-CMV152
Plasmid#179916PurposeAll in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.DepositorInsertCMV promoter sgRNA
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterU6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-sg-CMV51
Plasmid#179915PurposeAll in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 51.DepositorInsertCMV promoter sgRNA
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterU6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-sg-CMV162
Plasmid#179917PurposeAll in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.DepositorInsertCMV promoter sgRNA
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterU6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-sg-mGreenLantern332
Plasmid#179914PurposeAll in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.DepositorInsertmGreenLantern sgRNA
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterU6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-d80
Plasmid#114025PurposeInducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell linesDepositorInserteGFP-d80-HEC1 (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 1 to 80 deletedPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHJ33
Plasmid#48362PurposeTargets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin selectableDepositorInsertPartial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-loxP-3' ALD UTR
UseCre/Lox; Knockout in t. bruceiTagsExpressionMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pyrFEKO-PUR
Plasmid#24021DepositorTypeEmpty backboneUseFloxed targeting vector for trypanosomeTagsExpressionMutationPromoterAvailable sinceMay 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMOTag2T-crelox-PUR
Plasmid#24026DepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoterAvailable sinceFeb. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pPtPBR2
Plasmid#80387PurposeEpisome vector for Phaeodactylum tricornutum (pBR322-based)DepositorTypeEmpty backboneUseSynthetic Biology; ; episomal vector for phaeodac…TagsExpressionMutationPromoterAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only