pyrFEKO-PUR
(Plasmid
#24021)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24021 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepyrFEKO
- Backbone size (bp) 5753
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Vector typeFloxed targeting vector for Trypanosome
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI/PvuII (not destroyed)
- 3′ cloning site SbfI/HindIII (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Unpublished (variant of constructs described in Scahill et al 2008).
For more information about using this vector, please see: http://tryps.rockefeller.edu/trypsru2_cre-lox.html
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pyrFEKO-PUR was a gift from George Cross (Addgene plasmid # 24021 ; http://n2t.net/addgene:24021 ; RRID:Addgene_24021)