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Plasmid#133539PurposesfGFP driven by a T7 promoter with the tetO sequence 36 bp upstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.1
Plasmid#133523PurposesfGFP driven by a T7 promoter with the tetO sequence 4 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc'-cYAP-1
Plasmid#66854PurposeExpress Myc-tagged C.elegans YAP-1 in mammalian cellsDepositorInsertYAP-1 (yap-1 Nematode)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-SYNJ2BP-1
Plasmid#92159PurposeCRISPR guide RNA targeting human SYNJ2BPDepositorInsertSYNJ2BP sgRNA-1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-GFP
Plasmid#175363PurposeZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cellsDepositorInsertsgGFP
UseTagsExpressionInsectMutationF64L, S65C, and I167TPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-NBK-1
Plasmid#207639PurposeA plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.DepositorInsertPyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
UseTagsExpressionBacterialMutationThe evolved synthetase NBK-1 contains mutations Y…PromoteraraBADAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBluescript GPX-1
Plasmid#13038DepositorInsertGPX-1 (GPX1 Human)
UseTagsExpressionBacterialMutationAdditional GCG upstream of codon 11. Codon 92 is …PromoterAvailable sinceNov. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1 TIM8,13
Plasmid#170282PurposeExpresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertyeast Tim8 and Tim13 (TIM8 Budding Yeast)
UseTagsTEV-cleavable N-terminal His tag on Tim13 (on bac…ExpressionBacterialMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBXNPHM3-Nb_MsbA#1
Plasmid#186428PurposePlasmid for bacterial expression of MsbA binding Nanobody Nb_MsbA#1DepositorInsertNanobody Nb_MsbA#1
UseAffinity Reagent/ AntibodyTags3C cleavage site, His Tag, Maltose Binding Protei…ExpressionMutationPromoterpBADAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1371-sgITGB4-1
Plasmid#121535PurposesgITGB4-1 sequence: GAGGCGCAGTCCTTATCCACA. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.DepositorInsertsgITGB4-1
UseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromotermouse U6Available sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1371-sgITGB1-1
Plasmid#121533PurposesgITGB1-1 sequence: GAAGCAGGGCCAAATTGTGGG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.DepositorInsertsgITGB1-1
UseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromotermouse U6Available sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
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AAV.CBA.YFP.miR-E_shSrpk3-1
Plasmid#180400PurposeProducing AAV that encodes mouse Srpk3 shRNA-1 with miR-E backboneDepositorInsertshSrpk3-1 (Srpk3 Mouse)
UseAAV and RNAiTagsExpressionMutationPromoterCBAAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_DHN_TsDHN-1 (pBS0805)
Plasmid#185264PurposeFor the mammalian expression of the Eutrema salsugineum protein DHN_TsDHN-1. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertDHN_TsDHN-1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgCTRL-1
Plasmid#83934PurposeLentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.DepositorInsertsgCTRL-1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shStk16-1
Plasmid#180391PurposeProducing AAV that encodes mouse Stk16 shRNA-1 with miR-E backboneDepositorInsertshStk16-1 (Stk16 Mouse)
UseAAV and RNAiTagsExpressionMutationPromoterCBAAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-1 (GB1204)
Plasmid#75405PurposeProvides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid partDepositorInsertAtU6-1 promoter
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-WTS-1
Plasmid#66856PurposeExpress FLAG-tagged C.elegans WTS-1 in mammalian cellsDepositorInsertWTS-1 (wts-1 Nematode)
UseTagsFLAGExpressionMammalianMutationE153G and D871G mutations in WTS1 compared to Gen…PromoterCMVAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(S131C)
Plasmid#224744PurposeIn vitro transcription for Oocyte expression of mTREK-1(S131C)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131CPromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(S131A)
Plasmid#224745PurposeIn vitro transcription for Oocyte expression of mTREK-1(S131A)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131APromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only