pFLAG-WTS-1
(Plasmid
#66856)
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PurposeExpress FLAG-tagged C.elegans WTS-1 in mammalian cells
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 66856 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFLAG-CMV-2
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Backbone manufacturerEastman Kodak Company
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWTS-1
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SpeciesC. elegans (nematode)
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MutationE153G and D871G mutations in WTS1 compared to GenBank reference sequence NP_492699.1
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Entrez Genewts-1 (a.k.a. CELE_T20F10.1)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFLAG-WTS-1 was a gift from Yutaka Hata (Addgene plasmid # 66856 ; http://n2t.net/addgene:66856 ; RRID:Addgene_66856) -
For your References section:
Yes-associated protein homolog, YAP-1, is involved in the thermotolerance and aging in the nematode Caenorhabditis elegans. Iwasa H, Maimaiti S, Kuroyanagi H, Kawano S, Inami K, Timalsina S, Ikeda M, Nakagawa K, Hata Y. Exp Cell Res. 2013 Apr 15;319(7):931-45. doi: 10.1016/j.yexcr.2013.01.020. Epub 2013 Feb 7. 10.1016/j.yexcr.2013.01.020 PubMed 23396260