Skip to main content
Addgene

pFLAG-WTS-1
(Plasmid #66856)

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66856 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pFLAG-CMV-2
  • Backbone manufacturer
    Eastman Kodak Company
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WTS-1
  • Species
    C. elegans (nematode)
  • Mutation
    E153G and D871G mutations in WTS1 compared to GenBank reference sequence NP_492699.1
  • Entrez Gene
    wts-1 (a.k.a. CELE_T20F10.1)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-pA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLAG-WTS-1 was a gift from Yutaka Hata (Addgene plasmid # 66856 ; http://n2t.net/addgene:66856 ; RRID:Addgene_66856)
  • For your References section:

    Yes-associated protein homolog, YAP-1, is involved in the thermotolerance and aging in the nematode Caenorhabditis elegans. Iwasa H, Maimaiti S, Kuroyanagi H, Kawano S, Inami K, Timalsina S, Ikeda M, Nakagawa K, Hata Y. Exp Cell Res. 2013 Apr 15;319(7):931-45. doi: 10.1016/j.yexcr.2013.01.020. Epub 2013 Feb 7. 10.1016/j.yexcr.2013.01.020 PubMed 23396260