Addgene

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pBbA2c-RFP

Plasmid

#35326

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbE5a-RFP

Plasmid

#35277

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbB5k-GFP

Plasmid

#35354

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbE7c-RFP

Plasmid

#35314

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD_IPF2.0

Plasmid

#163124

Purpose

A plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.

Depositor

Julie Biteen

Article

Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

Insert

IFP2.0

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbE6c-RFP

Plasmid

#35287

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

LlacO-1

Available since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pBbE8k-RFP

Plasmid

#35270

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

BAD

Available since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-TTP(C147R)-GG-PUF

Plasmid

#52138

Purpose

Receiving vector for PUM-HD assembly and expression in mammalian cells

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Type

Empty backbone

Use

Tags

(GGGGS)3GGGG linker, 3xFlag, PUM-HD flanking regi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2014

Availability

Academic Institutions and Nonprofits only

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pGCG26

Plasmid

#82371

Purpose

YFP expressed from pJ23119 in the glpK locus with gentamicin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

YFP

Use

Tags

Expression

Mutation

Promoter

PJ23119

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBbE8c-RFP

Plasmid

#35269

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

BAD

Available since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ8069 - ACTB GFP-P2A + RAB11A mCherry-P2A CLIP Donor

Plasmid

#202689

Purpose

CLIP donor template to generate 2 knock-ins: GFP into ACTB, and mCherry in RAB11A.

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Inserts

CopGFP
mCherry

Use

Tags

Expression

Mutation

Promoter

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBEVY-4420-mCherry

Plasmid

#218828

Purpose

Yeast surface display of an anti-fluorescene scFv and intracellular expression of mCherry

Depositor

Yongku Cho

Article

Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

Insert

4-4-20 scFv

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pBEVY-pT231 scFv-GFP

Plasmid

#218827

Purpose

Yeast surface display of a phospho-tau pT231 scFv and intracellular expression of GFP

Depositor

Yongku Cho

Article

Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

Insert

pT231 tau scFv

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbS5k-RFP

Plasmid

#35285

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas49

Plasmid

#82391

Purpose

rbcL-sfGFP expressed from PccmK2 promoter with Gmr in glpK

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

rbcL-GFP

Use

Tags

Expression

Mutation

Promoter

PccmK2

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbS2k-RFP

Plasmid

#35330

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbS8k-RFP

Plasmid

#35276

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

BAD

Available since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbE0a-RFP

Plasmid

#35372

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 1, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCTCON2-GFP

Plasmid

#158130

Purpose

Single fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

sfGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbA0c-RFP

Plasmid

#35373

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 1, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge