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Plasmid#138666PurposeExpresses a mouse SIK1-targeting sgRNA, Cas9, and Cre-recombinaseDepositorInsertsgSIK1 mouse (Sik1 Mouse)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSECC-sgSIK1-A
Plasmid#138665PurposeExpresses a mouse SIK1-targeting sgRNA, Cas9, and Cre-recombinaseDepositorInsertsgSIK1 mouse (Sik1 Mouse)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSECC-sgMARK4-A
Plasmid#138676PurposeExpresses a mouse MARK4-targeting sgRNA, Cas9, and Cre-recombinaseDepositorInsertsgMARK4 mouse (Mark4 Mouse)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSECC-sgMARK4-B
Plasmid#138677PurposeExpresses a mouse MARK4-targeting sgRNA, Cas9, and Cre-recombinaseDepositorInsertsgMARK4 mouse (Mark4 Mouse)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSECC-sgSIK3-B
Plasmid#138673PurposeExpresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinaseDepositorInsertsgSIK3 mouse (Sik3 Mouse)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [ArchT-tdTomato]
Plasmid#123607PurposeAAV mediated expression of ArchT-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. tdTomato has codons varied to reduce recombination. Using bGHpA signal.DepositorInsertArchT-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterSynAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK141
Plasmid#73316PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tagDepositorInsertdelta-12 fatty acid acetylenase
UseTagsHis6, Pas2p signal sequence, and c-mycExpressionYeastMutationsynthetic genePromoterAOX1Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-HA-GFP
Plasmid#179912PurposeConstitutively secreted GFPDepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pJK276
Plasmid#73366PurposeEncodes Crepis alpina plastid omega-3 fatty acid desaturase (FAD7)DepositorInsertplastid omega-3 fatty acid desaturase
UseTagsExpressionYeastMutationMutation I231F. Also insert is missing residues 1…PromoterGAL1Available sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK142
Plasmid#73317PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tagDepositorInsertdelta-12 fatty acid acetylenase
UseTagsHis6, Pas2p, and c-mycExpressionYeastMutationsynthetic gene. Encodes a glycine-204 to serine m…PromoterAOX1Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-SEAP
Plasmid#179908PurposeSecreted Embryonic Alkaline PhosphataseDepositorInsertSEAP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-SEAP
Plasmid#231212PurposeSecreted Embryonic Alkaline PhosphataseDepositorInsertSEAP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
VanR-wt
Plasmid#153339PurposeWild type VanR from Caulobacter crescentus. The gene is under the control of the TEF1 promoterDepositorInsertVanR
UseTagsExpressionYeastMutationPromoterTEF1Available sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNGFP-BC
Plasmid#161956PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneUseTagsGFPuv His8ExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pCGFP-BC
Plasmid#161959PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneUseTagsGFPuv His8ExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pNGFP-EU
Plasmid#161967PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneUseTagsEGFP His8ExpressionMammalianMutationPromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCGFP-EU
Plasmid#161968PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneUseTagsEGFP His8ExpressionMammalianMutationPromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
HE_150
Bacterial Strain#201051PurposeDH10B-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures.DepositorBacterial ResistanceStreptomycinAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
HE_100
Bacterial Strain#201050PurposeDH10B-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures.DepositorBacterial ResistanceStreptomycinAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only