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Plasmid#169532PurposeTier-1 vector encoding PhCMV-driven iRFP670 that is localized to the nucleus (PhCMV-3xNLS-iRFP670-pA).DepositorInsertPCMV-driven nucleus-localized far-red fluorescent protein iRFP670
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pmyc-GFP-TNRC6A-del.GW-I+II+III-NES-mut
Plasmid#42040DepositorInsertTNRC6A (TNRC6A Human)
UseTagsEGFP and MycExpressionMammalianMutationdeleted aa457-532, aa716-772 and aa804-924, F951A…PromoterCMVAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
CMV-2xFLAG-Atoh1ER-DsRed
Plasmid#101735PurposeExpresses mouse Atoh1 fused to ER and DsRedDepositorInsertAtoh1 (Atoh1 Mouse)
UseTags2x FLAG, DsRed, and ERExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
aL-mEos3.2
Plasmid#113154Purposeconstrained LFA-1-mEos3.2 fusionDepositorInsertfull-length human aL with mEOS3.2 in betapropeller (ITGAL Human)
UseTagsmEOS3.2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
V5-WT-short-UBASH3A
Plasmid#192088PurposeMammalian expression of human wild-type short-form UBASH3A with V5 tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-ZapCY2
Plasmid#59016PurposeCytosolic zinc-binding FRET biosensorDepositorInsertNES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
UseTagsNuclear Exclusion SignalExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
mcherry Beta 3
Plasmid#36113DepositorInsertG protein beta subunit B3 (GNB3 Human)
UseTagsmcherryExpressionMammalianMutationPromotercmvAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
mcherry Beta 4
Plasmid#36114DepositorInsertG protein beta subunit B4 (Gnb4 Mouse)
UseTagsmcherryExpressionMammalianMutationPromotercmvAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
a5-ecto-pcDNA31hygro
Plasmid#113386PurposeExpress human alpha5 ectodomain with secretion peptide, purification tags and C-terminal claspDepositorInserthuman alpha 5 ectodomain
UseTagsStrep II tagExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTS2311-Tier1-spacer-PPGK-NLuc_TB
Plasmid#169552PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPPGK-NanoLuc Luciferase - mTagBFP fusion
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pActin-miRFP718nano
Plasmid#195745PurposeHuman beta-actin labelled with near-infrared fluorescent protein miRFP718nanoDepositorInsertmiRFP718nano-human beta-actin
UseTagsmiRFP718nanoExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPA-RL-FL
Plasmid#78863PurposeDULIP Tandem Construct used for data normalizationDepositorInsertFirefly
UseExpression vectorTagsProtein A, Renilla luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-ZapCmR1.1
Plasmid#59014PurposeCytosolic zinc-binding FRET biosensorDepositorInsertNES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
UseTagsNuclear Exclusion SignalExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 12
Plasmid#36109DepositorInsertG protein subunit Gamma 12 (Gng12 Mouse)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-BAP-Sox2
Plasmid#133281PurposeExpression of target proteins BAP-Sox2 in mammalian cellsDepositorInsertSox2 (SOX2 Human)
UseTagsBiotin Acceptor Peptide contains 7-His-tagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFL-V5-GW
Plasmid#78857PurposeDULIP Prey Destination Vector (Gateway, N-terminal)DepositorTypeEmpty backboneUseGateway destination vectorTagsFirefly luciferase, V5-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPA-RL-GW
Plasmid#78855PurposeDULIP Bait Destination Vector (Gateway, N-terminal)DepositorTypeEmpty backboneUseGateway destination vectorTagsProtein A-Renilla luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTS2381-Tier1-PhCMV-Igk_TM
Plasmid#169487PurposeTier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) for membrane localization of fusion proteins attached to a secretion signal Igk (PhCMV-IgkSS-TM-pA).DepositorInsertPCMV-driven membrane localization
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -