pcDNA3-BAP-Sox2
(Plasmid
#133281)
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PurposeExpression of target proteins BAP-Sox2 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133281 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
- Backbone size w/o insert (bp) 6894
- Total vector size (bp) 7850
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSox2
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Alt nameSox2
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SpeciesH. sapiens (human)
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Insert Size (bp)956
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GenBank IDNM_003106
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Entrez GeneSOX2 (a.k.a. ANOP3, MCOPS3)
- Promoter CMV
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Tag
/ Fusion Protein
- Biotin Acceptor Peptide contains 7-His-tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CTCTGGCTAACTAGAGAAC
- 3′ sequencing primer AGACGGCAATATGGTGGA (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
1. Kulyyassov, A. et al. PUB-MS: A Mass Spectrometry-based Method to Monitor Protein-Protein Proximity in vivo. Journal of Proteome Research10, 4416-4427, doi:10.1021/pr200189p (2011)
2. Shoaib, M. et al. PUB-NChIP-"in vivo biotinylation" approach to study chromatin in proximity to a protein of interest. Genome Research23, 331-340, doi:10.1101/gr.134874.111 (2013)..
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-BAP-Sox2 was a gift from Arman Kulyyassov (Addgene plasmid # 133281 ; http://n2t.net/addgene:133281 ; RRID:Addgene_133281) -
For your References section:
In Vivo Quantitative Estimation of DNA-Dependent Interaction of Sox2 and Oct4 Using BirA-Catalyzed Site-Specific Biotinylation. Kulyyassov A, Ogryzko V. Biomolecules. 2020 Jan 16;10(1). pii: biom10010142. doi: 10.3390/biom10010142. 10.3390/biom10010142 PubMed 31963153