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Plasmid#118229PurposeEmpty E. coli - B. burgdorferi shuttle vector; hygromycin B resistantDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only
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pgRNA-backbone
Plasmid#172517PurposeYeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertionDepositorInsertgRNA cassette
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSY6
Plasmid#90482PurposeTargeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicumDepositorInsertgroup II intron and ItrA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Clathrin-LSSmGFP
Plasmid#186537PurposeLocalization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to clathrinDepositorInsertLSSmGFP
UseTagsClathrinExpressionMammalianMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tom20-CIB-stop
Plasmid#117243PurposeLight activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switchDepositorInsertCIBN
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAT15489_dABE8e
Plasmid#174127Purposeplasmid expressing ABE8e with dCas9DepositorInsertdABE8e
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-I-N-CAAX
Plasmid#41686PurposeExpresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activationDepositorInsertDronpa145N-IntersectinDH-Dronpa145N-CAAX
UseTagsCAAX boxExpressionMammalianMutationK145N on DronpaPromoterCMVAvailable sinceDec. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK5-myc-PAT1
Plasmid#88856Purposemyc-TAG overexpressionDepositorInsertSLC36A1 (SLC36A1 Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBJ5 BRG1 DN
Plasmid#17874DepositorInsertBrg DN (SMARCA4 Human)
UseTagsExpressionMammalianMutationdominant negativePromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shLuc
Plasmid#83092PurposeLentiviral shRNA vector for inducible knockdown of luciferase (control hairpin)DepositorInsertshLuc
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
G783A_pLenti-Tet-BattP(GA)-miRFP670-2A-iCasp9-2A-Hygro_rtTA3
Plasmid#200635PurposeNegative selection hygromycin resistant Tet-inducible Matreyek Bxb1(GA) landing pad for dual landing pad cellsDepositorInsertiCasp9
UseLentiviralTagsExpressionMutationPromoterpTRE3GAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
VAChT-pHuji
Plasmid#105319PurposeVAChT tagged with pH-sensitive red fluorescent protein pHujiDepositorInsertVAChT (Slc18a3 Rat)
UseTagspHujiExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
TR-Csy4
Plasmid#80601PurposeExpresses Csy4 from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertCsy4
UseAAVTagsExpressionMammalianMutationPromoterCBAAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Plk4(Sak) D154A (Nigg HR26)
Plasmid#41164DepositorInsertPlk4 D154A (PLK4 Human)
UseTags3xMyc and PreScission SiteExpressionMammalianMutationD154APromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
YFP-ICP0FxE-C3
Plasmid#134561PurposeExpresses YFP-ICP0 fusion protein with a RING finger deletion (FXE)DepositorInsertICP0
UseTagsYFPExpressionMammalianMutationRING finger deletion (FXE)PromoterCMVAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAT15482_ABE8e
Plasmid#174120PurposeExpresses ABE8e with an EF1a promoterDepositorInserteABE
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCD017
Plasmid#177322PurposedCas9 expressionDepositorInsertdCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT005
Plasmid#177323PurposeMCP-SoxS (R93A) expressionDepositorInsertSoxS
UseTagsMCP (MS2 Coat Protein)ExpressionMutationR93APromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT009.J1.J306
Plasmid#177325PurposescRNA targeting J3 promoter for activationDepositorInsertJ306
UseTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT009.J3.RR2
Plasmid#177326PurposesgRNA targeting RFP for repressionDepositorInsertRR2
UseTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only