Addgene

We narrowed to 14,719 results for: LEA

Showing: 3521 - 3540 of 14719 results

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pNeoSagRNAH

Plasmid

#123266

Purpose

Expresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Type

Empty backbone

Use

CRISPR; Leishmania expression

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI119

Plasmid

#126059

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

chloroplastic FNR transit peptide

Use

Synthetic Biology; Module 0

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI120

Plasmid

#126060

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Synthetic Biology; Module 0

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD4

Plasmid

#121108

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (N601A) (PBRM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

PBRM1 (N601A)

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD5

Plasmid

#121109

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (N739A) (PBRM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

PBRM1 (N739A)

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD1

Plasmid

#121105

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (Y127A) (PBRM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

PBRM1 (Y127A)

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD3

Plasmid

#121107

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (N463A) (PBRM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

PBRM1 (N463A)

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD2

Plasmid

#121106

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (N263A) (PBRM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

PBRM1 (N263A)

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0114

Plasmid

#117553

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sgRNA_NbPDS1 N. benthamiana

Use

Tags

Expression

Plant

Mutation

Promoter

At U6-26 (pICSL90002)

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0029

Plasmid

#117541

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for LbCas12a, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

crRNA (LbCas12a)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0028

Plasmid

#117540

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for AsCas12a, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

crRNA (AsCas12a)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0014

Plasmid

#117524

Purpose

Level0 Golden Gate part: CDS, SpCas9-KA

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-KA (with stop codon)

Use

CRISPR

Tags

Expression

Mutation

K855A

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0009

Plasmid

#117522

Purpose

Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-p (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL90005

Plasmid

#117520

Purpose

Level0 Golden Gate part: CDS, SpCas9-h (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-h (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0008

Plasmid

#117535

Purpose

Level0 Golden Gate part: CDS, AsCas12a

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

AsCas12a (with stop codon)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mutation

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pMT3928

Plasmid

#113331

Purpose

control for Hermes transposition; LEU2 without flanking Hermes TIRs, recoded transposase driven by TEF1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

Inserts

beta-isopropyl malate dehydrogenase
codon-optimized Hermes transposase

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAW218

Plasmid

#113240

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS circfirefly_1100

Plasmid

#110117

Purpose

Expresses a 1100-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS circfirefly_900

Plasmid

#110116

Purpose

Expresses a 900-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS circfirefly_700

Plasmid

#110115

Purpose

Expresses a 700-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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