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pDO010

Plasmid

#198772

Purpose

Drives specific expression in the ALM, PLM, AVM, PVM neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pmec-17-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHW489

Plasmid

#198771

Purpose

Drives specific expression in the ALA neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pflp-24-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP018

Plasmid

#198766

Purpose

Drives specific expression in the ADE, CEP, and PDE neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pdat-1-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP097

Plasmid

#198790

Purpose

Co-expression plasmid for the CEP neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

PF09E10.7-NLS-gp41-1-C-intein-cGAL(AD)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP101

Plasmid

#198791

Purpose

Co-expression plasmid for the FLP, PVD neurons or the PHC neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pkcnl-4-NLS-cGAL(DBD)-gp41-1-N-intein

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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hsLEMD2-pX330

Plasmid

#179511

Purpose

Encodes gRNA for human LEMD2

Depositor

Yasushi Hiraoka

Article

Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

Insert

gRNA for LEMD2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLL5.0 CortW22A mCherry

Plasmid

#187268

Purpose

Lenticiral expression of mCherry-Cortactin W22A

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

Cortactin (CTTN Human)

Use

Lentiviral

Tags

mcherry

Expression

Mutation

W22A

Promoter

5' LTR

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCCM’ ΔacRAF

Plasmid

#162710

Purpose

Second CCM operon lacking acRAF; Deletion of putative rubisco chaperone, acRAF

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

Second CCM operon cloned from H. neapolitanus

Use

Tags

Expression

Bacterial

Mutation

pCCM' with a deletion of putative rubisco ch…

Promoter

PLtet0-1 promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTone-gata2aECE-EGFP

Plasmid

#132974

Purpose

gata2a endothelial enhancer (x6) and basal promoter driving egfp in pTol1 backbone

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Inserts

eGFP
gata2a endothelial enhancer (x6) with a carp b-actin basal promoter

Use

Unspecified; Transposon-mediated

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SNORD2 probe

Plasmid

#73070

Purpose

antisense probe for detection of human SNORD2 (C/D box snoRNA snR39B)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD2 (SNORD2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

t7

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_SNORD60 probe

Plasmid

#73071

Purpose

antisense probe for detection of human SNORD60 (C/D box snoRNA U60)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD60 (SNORD60 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

t7

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pGWB425

Plasmid

#74819

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223 PAK5 P50L

Plasmid

#60533

Purpose

Donor vector for human Pak5 P50L mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

P50L

Promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 P76T

Plasmid

#60534

Purpose

Donor vector for human Pak5 P76T mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

P76T

Promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 S312C

Plasmid

#60535

Purpose

Donor vector for human Pak5 S312C mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

S312C

Promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 V463L

Plasmid

#60536

Purpose

Donor vector for human Pak5 V463L mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

V463L

Promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 V593I

Plasmid

#60539

Purpose

Donor vector for human Pak5 V593I mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

V593I

Promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pGMEH-0X750-luxAB-DAS+4

Plasmid

#49519

Purpose

Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; replicates episomally

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

P750-luxAB-DAS+4

Use

Tags

DAS+4 (AANDENYSENYADAS)

Expression

Bacterial

Mutation

Promoter

P750

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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p6620 MSCV-IP N-HAonly AMPH

Plasmid

#35016

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

AMPH (AMPH Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

Feb. 9, 2012

Availability

Academic Institutions and Nonprofits only
pX330S-2-PITCh

Plasmid

#63670

Purpose

Expresses Cas9 nuclease and the PITCh-gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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