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Human T (BRACHYURY) sgRNA
Plasmid#59726PurposePlasmid encoding sgRNA to generate T (BRACHYURY) knock-out mutant human cellsDepositorInserthT sgRNA (TBXT Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro H-Ras G12V T35S
Plasmid#12274DepositorInsertH-Ras G12V T35S (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationContains G12V activating mutation. The T35S mutat…PromoterAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro H-Ras G12V Y40C
Plasmid#12276DepositorInsertH-Ras G12V Y40C (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationContains G12V activating mutation. The Y40C mutat…PromoterAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJP-HBB-nLuc
Plasmid#175431PurposeExpresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.DepositorInsertnLUC plus 5' HBB leader
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL Blast H-Ras G12V T35S
Plasmid#12278DepositorInsertH-Ras G12V T35S (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationContains G12V activating mutation. The T35S mutat…PromoterAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro H-Ras G12V E37G
Plasmid#12275DepositorInsertH-Ras G12V E37G (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationContains G12V activating mutation. The E37G mutat…PromoterAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pWZL Blast H-Ras G12V E37G
Plasmid#12277DepositorInsertH-Ras G12V E37G (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationContains G12V activating mutation. The E37G mutat…PromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pWZL Blast H-Ras G12V Y40C
Plasmid#12279DepositorInsertH-Ras G12V Y40C (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationContains G12V activating mutation. The Y40C mutat…PromoterAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcS-RDG-C1C2
Plasmid#200163PurposeThis plasmid encodes non-binder control monobody (RDG) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.DepositorInsertsRDG-C1C2
C1C2 domain of lactadherin
UseTagsHA and HISExpressionMammalianMutationsequence adopted from doi: 10.1371/journal.pone.0…PromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcS-E626-C1C2
Plasmid#200162PurposeThis plasmid encodes EGFR targeting monobody (E626) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.DepositorInsertsE626
C1C2 domain of lactadherin
UseTagsHA and HISExpressionMammalianMutationsequence adopted from doi: 10.1371/journal.pone.0…PromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-2P-Omicron-BA.5
Plasmid#213828PurposeMammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.2P.BA5 variantDepositorInsertpαH-S-GSAS/Omicron-2P-BA5 (S )
UseTagsHRV 3C cleavage site (before tags) 8X His tag 2X…ExpressionMammalianMutationG339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
5HT6-YFP-Inpp5e
Plasmid#96808Purposecilia-membrane-targeted catalytic domain of inositol phosphae 5-phosphatase, CAAX-nullDepositorInsert5HT6-YFP-Inpp5e
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMIR218-2_nt5G>U
Plasmid#174009PurposemiR218 nt5 point mutation in SLIT3DepositorUseTagsExpressionMammalianMutationNT5 G>U point mutation in SLIT3PromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJAM1.20_PB-NEDD4L_deltaC2-PGK-Blast
Plasmid#205565PurposeExpress human NEDD4L (delta C2 isoform)DepositorInsertNEDD4L (NEDD4L Human)
UsePiggybacTagsExpressionMammalianMutationdelta C2 isoformPromoterEF1Available sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pArk313
Plasmid#200257PurposeArk313 rep/capDepositorInsertArk313 Rep/Cap
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTSin EGFP
Plasmid#127695PurposePlasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequenceDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSRE SSG
Plasmid#127692PurposePlasmid expresses the Sindbis Structural Genome (SSG) via the serum response element (SRE) promoter. See Resource Information section for annotated plasmid sequence.DepositorInsertSindbis Structural Genome
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
V5-LOV-Turbo1-G3BP1_pTRE-G418
Plasmid#202015Purposeexpresses LOV-Turbo1-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vectorDepositorInsertV5-LOV-Turbo1-G3BP1
UseLentiviralTagsV5ExpressionMammalianMutationPromoterpTRE-TightAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
V5-LOV-Turbo1-NIK3x_pTRE-G418
Plasmid#202017Purposeexpresses LOV-Turbo1-NIK3x in the mammalian nucleolus under dox inducible promoter, lentiviral vectorDepositorInsertV5-LOV-Turbo1-NIK3x
UseLentiviralTagsV5ExpressionMammalianMutationPromoterpTRE-TightAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only