pMIR218-2_nt5G>U
(Plasmid #174009)

• Purpose: miR218 nt5 point mutation in SLIT3
• Depositing Lab: Andrew M. Siedlecki
• Publication: Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582. doi: 10.1681/ASN.2021020140. Epub 2022 Jan 28.

Backbone

• Vector backbone: pUC-GW-Kan
• Backbone size w/o insert (bp): 2626
• Total vector size (bp): 2811
• Vector type: Mammalian Expression
• Selectable markers: Kanamycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SLIT3
• Alt name: Mir218 with NT5 point mutation in SLIT3
• Species: H. sapiens (human)
• Insert Size (bp): 205
• Mutation: NT5 G>U point mutation in SLIT3
• GenBank ID: AY358884.1
• Entrez Gene: SLIT3 (a.k.a. MEGF5, SLIL2, SLIT1, Slit-3, slit2)
• Entrez Gene: MIR218-2 (a.k.a. MIRN218-2, mir-218-2)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13 Forward
• 3′ sequencing primer: M13 Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Genewiz, Inc.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry