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Plasmid#139776PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the pH promoter.DepositorInsertC-terminal 10His tag
UseTags10 HisExpressionInsectMutationPromoterPHAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-p10-Flag-Cter(VE5738)
Plasmid#161798PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the p10 promoter.DepositorInsertC-terminal Flag tag
UseTagsFlagExpressionInsectMutationPromoterp10Available sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_PH-10His-Nter-GWs-Lox (VE5588)
Plasmid#161806PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 10 His tag under the pH promoter.DepositorInsertN-terminal 10His tag
UseTags10 HisExpressionInsectMutationPromoterPHAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-p10-mCh PH-mCh (VE5625)
Plasmid#139769PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an mCherry cDNA under each promoter.DepositorInsertmCherry fluorescent protein
UseTagsExpressionInsectMutationPromoterPH or p10Available sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-IRESMuro
Plasmid#82336PurposeBase vector targeting genes to the GAPDH locus of human cells. Inserted genes are expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertIRESMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-ANLN
Plasmid#31203DepositorInsertANLN (ANLN Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS DV DNM1-EGFP
Plasmid#107794Purposedonor vector for knock-in gene editing at human DNM1 locusDepositorInsertDNM1-eGFP (DNM1 Synthetic, Human)
UseYeast-e.coli shuttle vectorTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoter
Plasmid#121813PurposepMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.DepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
nes1852tk/lacZ
Plasmid#47613Purposeused to create transgenic mice expressing LacZ reporter with Nestin enhancerDepositorInsertnestin 2nd intron (NES Human)
UseEnhancer reporterTagsHSV tk promoter and lacZExpressionMutation2nd intron onlyPromoterHSV tk promoterAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-LITAF
Plasmid#178444Purposedoxycycline-inducible expression of Human LITAF in mammalian cellsDepositorInsertLITAF (LITAF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-cpVenus-FLARE-AKAR
Plasmid#123329PurposeYellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.DepositorInsertVenus-cpVenus-FLARE-AKAR
UseTags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-mtagtdTom_IRESMuro
Plasmid#82355PurposeVector targeting the mTagtandem tomato gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertmtagTandemTomato-IRESMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-FLAG-IRES-blasticidin-Sox2 S248A
Plasmid#182047Purposeexpression of Sox2 mutantsDepositorInsertSox2 (Sox2 Mouse)
UseTagsFLAGExpressionMammalianMutationS248APromoterCAGAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A
Plasmid#182049Purposeexpression of Sox2 mutantsDepositorInsertSox2 (Sox2 Mouse)
UseTagsFLAGExpressionMammalianMutationS248A/T258APromoterCAGAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 T258A
Plasmid#182048Purposeexpression of Sox2 mutantsDepositorInsertSox2 (Sox2 Mouse)
UseTagsFLAGExpressionMammalianMutationT258APromoterCAGAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
KA801: pMVP (L3-L2) HA tag + pA; CMV::eGFP-P2A-TETa-pA
Plasmid#121800PurposepMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven GFP-P2A-TETaDepositorInsertHA epitope tag-polyA + CMV::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter
Plasmid#121819PurposepMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestionDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
RCAS-sgATG7
Plasmid#75228PurposeAn adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor GammohDepositorInsertAutophagy Related 7 (Atg7 Mouse)
UseRetroviralTagsExpressionMutationPromoterU6Available sinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
MI-Luciferase
Plasmid#75018PurposeEncodes the expression of Luciferase (with out any fluorescent reporter)DepositorInsertfirefly Luciferase
UseLuciferase and RetroviralTagsExpressionMutationPromoterAvailable sinceMay 31, 2016AvailabilityAcademic Institutions and Nonprofits only