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Plasmid#163671PurposeExpresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, TMD from ST6GAL1 and luminal domain from TFR in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the 9 amino acids from the …PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only
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F9FS-GFP
Plasmid#163672PurposeExpresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, TMD from TFR and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the 9 amino acids from the …PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2 gRNA resistant
Plasmid#163408PurposeExpresses BMPR-2 resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationgRNA-targeting sequences are substituted with nuc…PromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔEC gRNA resistant
Plasmid#163413PurposeExpresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe extracellular domain (27-150 aa) is deleted. …PromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔTail gRNA resistant
Plasmid#163412PurposeExpresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe C-terminal tail domain (530-1038 aa) is delet…PromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔKinase gRNA resistant
Plasmid#163411PurposeExpresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe kinase domain (202-500 aa) is deleted. gRNA-t…PromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV-Blast mLST8-Flag Mut2
Plasmid#124917PurposeFor stable expression_ sgRNA resistant Flag tagging mLST8 W272F/W274F mutantDepositorInsertMLST8 (MLST8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationInsert ORF was sirently mutated to be resistent t…PromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
MOK gRNA (BRDN0001145525)
Plasmid#75936Purpose3rd generation lentiviral gRNA plasmid targeting human MOKDepositorInsertMOK (MOK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MOK gRNA (BRDN0001148493)
Plasmid#75938Purpose3rd generation lentiviral gRNA plasmid targeting human MOKDepositorInsertMOK (MOK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
JA1280
Plasmid#49969PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD HB-10 (HBB Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
JA1266
Plasmid#49966PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD HB-7 (HBB Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
JA1229
Plasmid#49955PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD HB-2 (HBB Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
JA1217
Plasmid#49954PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD HB-1 (HBB Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (AAV1)
Viral Prep#75470-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (#75470). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry plasmid DNA. CAG-driven, Flp recombinase-dependent expression of channelrhodopsin H134R mutant fused to mCherry. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmCherryAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (AAV5)
Viral Prep#112005-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (#112005). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 plasmid DNA. Synapsin-driven, axon-targeted GCaMP6s calcium sensor and mRuby3 expression. The mRuby3 will be a separate protein (not a fusion protein). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmRuby3Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (AAV1)
Viral Prep#50942-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (#50942). In addition to the viral particles, you will also receive purified pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate mRuby2 expression under a human synapsin1 promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmRuby2Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (AAV9)
Viral Prep#112005-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (#112005). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 plasmid DNA. Synapsin-driven, axon-targeted GCaMP6s calcium sensor and mRuby3 expression. The mRuby3 will be a separate protein (not a fusion protein). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmRuby3Available sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2 (AAV9)
Viral Prep#112004-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2 (#112004). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2 plasmid DNA. Synapsin-driven, axon-targeted GCaMP6s calcium sensor and bicistronic mKate2 expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmKate2Available sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA (AAV1)
Viral Prep#68719-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA (#68719). In addition to the viral particles, you will also receive purified pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA plasmid DNA. CAG-driven, Cre-dependent GCaMP6f calcium sensor with bicistronic mRuby2. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAG-FLEXTagsmRuby2 (Cre-dependent)Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (AAV1)
Viral Prep#68717-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (#68717). In addition to the viral particles, you will also receive purified pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA plasmid DNA. CAG-driven, Cre dependent, GCaMP6s calcium sensor and bicistronic, physically separate mRuby2 expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAG-FLEXTagsmRuby2 (Cre-dependent)Available sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only