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Plasmid#61424PurposesgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only
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p5154
Plasmid#175390PurposeHigh Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)DepositorTypeEmpty backboneUseMouse Targeting; Cloning vector, to be used to de…TagsExpressionMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLTR.gp140/EGFP.Revdelta38/DsRed
Plasmid#115775PurposeHIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.DepositorInsertEnvelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
UseTagsEGFP; dsReDExpressionMammalianMutationgp140 uncleaved (1190-91aa, KR > TG; truncated…PromoterHIV-1 (LTR)Available sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
iCAP-ROOT
Plasmid#192262PurposeAAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsidDepositorInsertSynthetic construct for ROOT VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6T7
Plasmid#71462PurposeExpresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and MammalianMutationPromoterhuman U6/phage T7Available sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDAS12489_PEAR-GFP_2in1_2.0
Plasmid#177186PurposePEAR-GFP3 plasmid with a pegRNA that targets its own plasmidDepositorInsertsEGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid
UseCRISPRTagsExpressionMammalianMutationdisrupted 5' splice sitePromoterCMV and U6Available sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFPE9
Plasmid#83545PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.DepositorInsertTEF1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRepair-MPP8_mAID-P2A-BFP
Plasmid#175036PurposeHomology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9DepositorInsertmAID-P2A-BFP
UseHdr donor templateTagsExpressionMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-oNGN2
Plasmid#198397PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.InsertsUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationS24A, S193A, S207A, S209A, S219A, S232A, S239A, S…PromoterCAG, EF1a, and TRE3GAvailable sinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL0527 (pDonor)
Plasmid#130634PurposeEncodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (Mini-Tn)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-47: CTNNB1-mEGFP
Plasmid#109119PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human CTNNB1DepositorInsertCTNNB1 Homology Arms with mEGFP-linker (CTNNB1 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMay 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
Intron-Tagging-EGFP-Donor
Plasmid#159740PurposeContains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.DepositorInsertEGFP
UseIntron tagging donorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
L1_Cas9-Ck4
Plasmid#136135PurposeL1 in position 4, for AtcoCas9 (Puchta) expression in L2 CRISPR vectorsDepositorInsertp5-MpEF1a:Cas9_3tNos-35S
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
TC1316
Plasmid#164883PurposeCMV-bpNLS-dCas13d-bpNLS-ADARdd-mCherry, ADARdd embeded in loop3DepositorInsertdcas13d-ADARdd
UseTagsExpressionMammalianMutationdCas13d L560-G586 deletedPromoterCMV, T7Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
v1em-Cterm-PE2max-U6-pegRNA
Plasmid#198733PurposeAAV genome encoding C-terminal PE2max and U6 expression cassetteDepositorInsertNpuC-CtermPE2max
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
TC1681
Plasmid#164872PurposeCURE-C2: C to U RNA Editing, with high efficiency and high off-target effectsDepositorInserthAPOBEC3A fused to PspCas13b (APOBEC3A )
UseCRISPRTagsExpressionMammalianMutationhuman apobec Y132DPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRAC_DARIC(2-3)_CD22-BBz_AAV6_Donor
Plasmid#211906PurposeVector for AAV6 donor production. Targeted CD22-BBz DARIC transgene integration to the TRAC locus via homology-directed repair.DepositorInsertDARIC-CD22-BBz
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_VPR_yl
Plasmid#107677PurposeCRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertionDepositorInsertsCodon optimized dCas9-VPR
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
TC1332
Plasmid#164874PurposeCURE-N: C to U RNA Editing, with high efficiency and low off-target effectsDepositorInserthAPOBEC3A fused to PspCas13b (APOBEC3A )
UseCRISPRTagsExpressionMammalianMutationhuman apobec Y132DPromoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
PHP.AX
Plasmid#195218PurposeAAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)DepositorInsertSynthetic construct isolate AAV-PHP.AX VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only