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Plasmid#51253PurposeEncodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to AlaDepositorInsertGli2 (Gli2 Mouse)
UseFlpin systemTags3xHA-TEVExpressionMammalianMutationamino acids 923,956 mutated to AlaPromoterEF1aAvailable sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-ECFP
Plasmid#48353PurposeContributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertECFP
UseGateway entry vectorTagsExpressionMutationContains a stop codon.PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-EYFP
Plasmid#48351PurposeContributes the coding sequence for EYFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertEYFP
UseGateway entry vectorTagsExpressionMutationContains a stop codon.PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJA252
Plasmid#21512DepositorInsertpmex-5 (w/o ATG)
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJA256
Plasmid#21509DepositorInsert(Gly)5Ala::GFP(w introns)::tbb-2 3'UTR
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJA269
Plasmid#21544DepositorInsertphsp16.41::GFP-F64LS65T::(Gly)5Ala
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJA245
Plasmid#21506DepositorInsertpmex-5::GFP (w introns)::(Gly)5Ala
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJA257
Plasmid#21510DepositorInsert(Gly)5Ala::GFP-F64LS65T::tbb-2 3'UTR
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJA254
Plasmid#21507DepositorInsertpmex-5::GFP-F64LS65T::(Gly)5Ala
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJA255
Plasmid#21508DepositorInsertpmex-5::citrine (w introns)::(Gly)5Ala
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJA258
Plasmid#21511DepositorInsert(Gly)5Ala::citrine(w introns)::tbb-2 3'UTR
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
Ash1
Plasmid#169711PurposeBacterial Expression of yeast Ash1 truncation (encodes amino acids 420-500)DepositorInsertAsh1 (ASH1 Budding Yeast)
UseTags6xHisExpressionBacterialMutationTruncation including encoding amino acids 420-500PromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR P4-P1R-EYFP
Plasmid#48350PurposeContributes the coding sequence for EYFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertEYFP
UseGateway entry vectorTagsExpressionMutationContains a Kozak sequence. Does not contain a sto…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-SV40 polyA signal
Plasmid#48627PurposeContributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.DepositorInsertSV40 early polyadenylation signal cassette
UseGateway entry vectorTagsExpressionMutationPromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-mKate2
Plasmid#48345PurposeContributes the coding sequence for the mKate2 fluorescent protein as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertmKate2
UseGateway entry vectorTagsExpressionMutationContains a stop codon at the 3' end of the c…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P4-P1R-mKate2
Plasmid#48344PurposeContributes the coding sequence for the mKate2 fluorescent protein as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertmKate2
UseGateway entry vectorTagsExpressionMutationIt contains a Kozak sequence but no stop codon.PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P4-P1R-ECFP
Plasmid#48352PurposeContributes the coding sequence for ECFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertECFP
UseGateway entry vectorTagsExpressionMutationContains a Kozak sequence. Does not contain a sto…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR5'_ubi:loxP-EGFP-loxP
Plasmid#27322DepositorInsertubiquitin:loxP-EGFP-loxP
UseMultisite gateway 5' entry vectorTagsExpressionMutationPromoterAvailable sinceApril 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENTR eGFPCreERT2 L1-R5
Plasmid#43961DepositorInserteGFP-CreERT2
UseMultisite gateway entr cloneTagsExpressionMutationPromoterAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRL8
Plasmid#24980DepositorInsertCbr-unc-119
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only