pDONR P4-P1R-EYFP
(Plasmid
#48350)
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PurposeContributes the coding sequence for EYFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48350 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONRP4-P1R
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Backbone manufacturerLife Technologies/Invitrogen
- Backbone size w/o insert (bp) 2655
- Total vector size (bp) 3363
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Vector typeGateway entry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEYFP
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SpeciesSynthetic; Aequorea victoria
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Insert Size (bp)708
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MutationContains a Kozak sequence. Does not contain a stop codon.
- Promoter none
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 forward (-20)
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byClontech
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR P4-P1R-EYFP was a gift from Anna Planas & Tomas Santalucia (Addgene plasmid # 48350 ; http://n2t.net/addgene:48350 ; RRID:Addgene_48350) -
For your References section:
A plasmid toolkit for cloning chimeric cDNAs encoding customized fusion proteins into any Gateway destination expression vector. Buj R, Iglesias N, Planas AM, Santalucia T. BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18. 10.1186/1471-2199-14-18 PubMed 23957834