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mAG-GAL3

Plasmid

#62734

Purpose

Mammalian vesicle leakage reporter

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

mAG-Galectin 3

Use

Lentiviral

Tags

mAzami-Green

Expression

Mammalian

Mutation

Promoter

EF1-Alpha

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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FLX1.8NFATGFPpd2HS4

Plasmid

#169096

Purpose

Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation

Depositor

Geoffrey Owens

Inserts

NFAT enhancer (from IL-4 promoter)
Destabilized green fluorescent protein

Use

Lentiviral

Tags

Expression

Mutation

Promoter

NFAT enhancer + synthetic TATA box

Available since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMSP1E3D1

Plasmid

#20066

Purpose

Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

Insert

MSP1E3D1

Use

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeats of …

Promoter

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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mCherry-EB1-8

Plasmid

#55035

Purpose

Localization: MT End Binding Protein, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

EB1 (MAPRE1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pBABE Hygro Flag hTERT

Plasmid

#53546

Purpose

retroviral expression of human TERT

Depositor

Kathleen Collins

Article

Sexton et al Mol Cell Biol. 2011 Feb;31(4):736-43. do

Insert

TERT (TERT Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pmCherry-Gal3

Plasmid

#85662

Purpose

Fluorescent reporter for lysosomal damage

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

LGALS3 (LGALS3 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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ERK-KTR-mStrawberry reporter

Plasmid

#158686

Purpose

This plasmid is a ERK pathway reporter. It has CMV promoter driving the expression of ERK-KTR fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-EGFP-WPREpA

Plasmid

#84469

Purpose

pAAV-RAM-EGFP

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

TRE

Available since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-dCas9-KRAB-blast

Plasmid

#89567

Purpose

Plasmid expression dCas9 protein in fusion with KRAB domain

Depositor

Gary Hon

Article

Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

Insert

dCas9

Use

Lentiviral

Tags

Blasticidin resistance gene and KRAB

Expression

Mutation

D10A and H840A

Promoter

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-3xFLAG-V5-ccdB

Plasmid

#87072

Purpose

Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-SOX9

Plasmid

#141404

Purpose

Inducible Expression of SOX9

Depositor

Lorenz Studer

Article

Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

Insert

SOX9 (SOX9 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-puro

Plasmid

#45561

Depositor

Michael McVoy

Article

Abbate et al Biotechniques. 2001 Aug;31(2):336-40.

Insert

Puromycin N-acetyl transferase

Use

Bifunctional selection protein

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Phcmv (Strong human cytomegalovirus immediate ear…

Available since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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PKA-KTR-mStrawberry reporter

Plasmid

#158688

Purpose

This plasmid is a PKA pathway reporter. It has CMV promoter driving the expression of PKA-KTR fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pEF-GFP

Plasmid

#11154

Purpose

Mammalian expression vector for expression of GFP (EF1a promoter)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

EF1 alpha promoter (EEF1A1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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human p53-(1-393)

Plasmid

#24859

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Full length.

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pYPQ230-D156R (ttLbCas12a)

Plasmid

#195366

Purpose

A temperature-tolerant LbCas12a mutant (D156R), also known as ttLbCas12a

Depositor

Yiping Qi

Article

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

LbCas12a-D156R (ttLbCas12a)

Use

CRISPR; Gateway compatible lbcas12a-d156r (ttlbca…

Tags

Expression

Plant

Mutation

Promoter

ZmUBI1

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAC-SP6

Plasmid

#102418

Purpose

Ac transposase with SP6 promoter for in vitro transcription

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

Ac transposase

Use

Tags

Expression

Bacterial

Mutation

Promoter

SP6

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDisplay-BirA-ER

Plasmid

#20856

Depositor

Alice Ting

Article

Howarth et al Nat Methods. 2008 May . 5(5):397-9.

Insert

BirA-ER

Use

Tags

Expression

Mammalian

Mutation

C-terminal ER targeting sequence (KDEL)

Promoter

Available since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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pMK286 (mAID-NeoR)

Plasmid

#72824

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Neo

Use

CRISPR

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCRIS-PITChv2-Puro-dTAG (BRD4)

Plasmid

#91793

Purpose

PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

Puro-P2A-2xHA-FKBP_F36V

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

Promotorless

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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