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Plasmid#212806PurposeConstitutive or doxycycline-inducible expression of budding yeast Ubc13 in mammalian cellsDepositorInsertUbc13
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Mms2
Plasmid#212807PurposeConstitutive or doxycycline-inducible expression of budding yeast Mms2 in mammalian cellsDepositorInsertMms2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hALDOA K230A
Plasmid#210811PurposeTo block the formation of the initial Schiff base with FBPDepositorInsertALDOA (ALDOA Human)
UseTagsHAExpressionMammalianMutationresidues 230 of ALDOA mutated to alaninePromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA TfR1-PuRRRE
Plasmid#208327PurposeC-terminal PuRRRE-tagged transferrin receptor 1 (tag on extracellular domain).DepositorInsertTfR1-PuRRRE
UseTagsPuRRREExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA TfR1-TREER
Plasmid#208325PurposeC-terminal TREER-tagged transferrin receptor 1 (tag on extracellular domain).DepositorInsertTfR1-TREER
UseTagsTREERExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA TfR1-TinyERRE
Plasmid#208324PurposeC-terminal TinyERRE-tagged transferrin receptor 1 (tag on extracellular domain).DepositorInsertTfR1-TinyERRE
UseTagsTinyERREExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-N
Plasmid#210270PurposeN-terminal EGFP-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.DepositorTypeEmpty backboneUseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
cacna2d1 BBS pcDNA3
Plasmid#206106Purposeexpression of rat alpha2delta-1 calcium channel auxillary subunit with a single Bungarotoxin Binding Site motif inserted after aa549HADepositorInsertcacna2d1 (Cacna2d1 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi3-91-NLuc
Plasmid#207150PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAI3 (GNAI3 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)KPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoA-91-NLuc
Plasmid#207151PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)GPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoB-91-NLuc
Plasmid#207152PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)GPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA TfR1-PunyEEER
Plasmid#208326PurposeC-terminal PunyEEER-tagged transferrin receptor 1 (tag on extracellular domain).DepositorInsertTfR1-PunyEEER
UseTagsPunyEEERExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_ATP10B-G671R-N865K
Plasmid#203699Purposeexpresses ATP10B with the pathogenic mutations G671R and N865KDepositorInsertATP10B (ATP10B Human)
UseTagsExpressionMammalianMutationG671R, N865KPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GpACR1-pcDNA3.1-mScarlet
Plasmid#204964PurposeExpression of GpACR1 in fusion with mScarlet in mammalian cellsDepositorInsertGpACR1
UseTagsmScarletExpressionMammalianMutationPromoterCMV (+ enhancer)Available sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 W391A pcDNA3
Plasmid#206085Purposeexpression of rabbit Cav2.2 calcium channel with a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit bindingDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationW391APromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hARFRP1(T31N)-HA
Plasmid#206401PurposeMammalian expression of hARFRP1(T31N)DepositorInsertARFRP1 (ARFRP1 Human)
UseTagsHAExpressionMammalianMutationT31NPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hARFRP1(Q79L)-HA
Plasmid#206400PurposeMammalian expression of hARFRP1(Q79L)DepositorInsertARFRP1 (ARFRP1 Human)
UseTagsHAExpressionMammalianMutationQ79LPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+FLAG-KrasnatQ61R
Plasmid#206843PurposeTo express mouse Kras encoded by native mouse codons and with a Q61R mutation with an N-terminal FLAG tag.DepositorInsertKras (Kras Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Glutamine 61 to ArgininePromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2-Venus
Plasmid#203712PurposeConstitutive mammaliam expression of human NKX6-2 cDNA fused with the Venus fluorescent proteinDepositorInsertNKX6-2 fused with Venus (NKX6-2 Human)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2(1-40)-Venus
Plasmid#203713PurposeSPAX8-related truncated mutation (K41*) containing the amino acids 1 to 40 of human NKX6-2, fused with the Venus fluorescent protein.DepositorInsertNKX6-2 (1-40) fused with Venus (NKX6-2 Human)
UseTagsVenusExpressionMammalianMutationdeletion of amino acids 41-277PromoterCMVAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only