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Plasmid#13799DepositorInsertNRAS (NRAS Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPKm-118
Plasmid#90491PurposeExpresses pcDNA3 - CMVmin - 5X UAS - pFR LuciferaseDepositorInsertpFR Luciferase Fluc
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMEH305 HA NLS SpCas9 3x high affinity MS2 loops
Plasmid#168216PurposeMammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tagDepositorInsertSV40 NLS SpCas9
UseLentiviralTagsHA, SV40 NLS, and nucleoplasmin NLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-TREX-GFP-FF3
Plasmid#11667Purpose3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a Tet-responsive promoter (TREX) controlling expression of GFP and miR30-based shRNA targeting firefly luciferaseDepositorInsertFF3
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTS2311-Tier1-spacer-PPGK-NLuc_TB
Plasmid#169552PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPPGK-NanoLuc Luciferase - mTagBFP fusion
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5
Plasmid#125227PurposeExpresses SPARK DRD1 transmembrane component in mammalian cellsDepositorInsertDRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5
UseAAVTagsFlag, V5ExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4 mIL-17 1.1kb promoter
Plasmid#20125DepositorInsertmIL-17 (Il17a Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
-111 hTF m1
Plasmid#15448DepositorInsertTissue factor promoter (F3 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCheck2 Dest (R1-R2) #BGV129
Plasmid#48955PurposeDestination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.DepositorTypeEmpty backboneUseLuciferase; Gateway cloning vectorTagsExpressionMutationPromoterSV40Available sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB784
Plasmid#60163PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter EtsB site mutant pGL3Basic
Plasmid#32730DepositorInsertCCND1 (CCND1 Human)
UseLuciferaseTagsExpressionMutation-778 EtsB binding site AGGAA deletionPromoter-962 CCND1Available sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter EtsC site mutant pGL3Basic
Plasmid#32731DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-424 EtsC site TTCCT deletionPromoter-962 CCND1 promoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
p.UTA.2.0-GL-2
Plasmid#82440PurposeDual Fluorescent endogenous miRNA sensor. Perfect complementary region of an siRNA control targeting firefly luciferase encoded by the pGL2DepositorInsertLuciferase
UseTagsExpressionMutationThe complementary region for GL2 siRNA control wa…PromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
p6xAP-1_RE
Plasmid#118054PurposeGBPart - Operator/DNA Response element - 6 copies of the AP-1 binding motifDepositorInsert6 copies of the AP-1 binding motif
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (1)(2) sites mutant pGL3Basic
Plasmid#32734DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-75 TCF(1) and -68 TCF(2) - CTTTGATCTTTGCT deleti…Promoter-962 CCND1Available sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
Non-targeting CRISPRi plasmid
Plasmid#154891PurposeHuman non-targeting CRISPRi gRNADepositorInsertNontargeting gRNA
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Control-E2F-3'UTR-Mut
Plasmid#21171DepositorInsertE2F 3'UTR Mutant (E2F1 Human)
UseLuciferaseTagsExpressionMammalianMutation2 miR binding sites mutated. Changed from GCACTTT…PromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
Luc-Nanog-3'UTR
Plasmid#63893PurposeLuciferase reporter of mouse wildtype Nanog 3'UTRDepositorInsertNanog homeobox (Nanog Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG176 SLC23A2 3'UTR mut
Plasmid#12034DepositorInsertN9 3'UTR mut (SLC23A2 Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Akt2(K14A)
Plasmid#86624PurposeExpresses mCherry-tagged human Akt2(K14A) membrane binding mutantDepositorInsertAkt2(K14A) (AKT2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only