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Plasmid#178441Purposedoxycycline-inducible expression of Mouse Junb in mammalian cellsDepositorInsertJunb (Junb Mouse)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-NMI
Plasmid#178446Purposedoxycycline-inducible expression of Human NMI in mammalian cellsDepositorInsertNMI (NMI Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-TFEC
Plasmid#178447Purposedoxycycline-inducible expression of Human TFEC in mammalian cellsDepositorInsertTFEC (TFEC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-BATF2
Plasmid#178453Purposedoxycycline-inducible expression of Human BATF2 in mammalian cellsDepositorInsertBATF2 (BATF2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX_HA-C7orf26v1
Plasmid#178535PurposeSubcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into pLX_HA backboneDepositorInsertC7orf26v1 (INTS15 Human)
UseTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)
Plasmid#123340PurposeNegative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.DepositorInsertVenus-cpVenus-FLARE-EKAR-EV(T/A)
UseTags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-mCherry-FLARE-CKAR
Plasmid#123345PurposeRed-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.DepositorInsertmCherry-mCherry-FLARE-CKAR
UseTags6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cerulean3-Cerulean3-FLARE-AKAR
Plasmid#123335PurposeCyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.DepositorInsertCerulean3-Cerulean3-FLARE-AKAR
UseTags6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSR357.12
Plasmid#125107PurposeExpression of narL(1-134aa)-ydfI(129-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)134
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationA84T - see depositor comments below and Q183K-see…PromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
KL001: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-VPR
Plasmid#121836PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS/VPR scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sitesDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KL101: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-KRAB
Plasmid#121837PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS/KRAB scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sitesDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KL202: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-LSD1
Plasmid#121838PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS/LSD1 scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sitesDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KL401: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-Dnmt3a3L
Plasmid#121840PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS/Dnmt3a-3L scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sitesDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA
Plasmid#121803PurposepMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.DepositorInsertpolyA + EF1a::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pA
Plasmid#121807PurposepMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest.DepositorInsertpolyA + RIP::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LF901: pMAGIC (L1-R5) mU6::SaCas9 gRNA scaffold
Plasmid#121811PurposepMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.DepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JI601: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; RIP
Plasmid#121815PurposepMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.DepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JI702: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; RIP
Plasmid#121820PurposepMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.DepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only